Re: [ccp4bb] Nobel Prizes for 3D Molecular Structure

2012-10-13 Thread Joel Sussman
Just want to make sure anyone interested in Nobel Prizes knows about this existing page: http://proteopedia.org/wiki/index.php/Nobel_Prizes_for_3D_Molecular_Structure best regards, Joel Sussman

Re: [ccp4bb] CCP4 update 6.3.0 006

2012-10-13 Thread Dmitry Rodionov
Hi Ben, Applications launched from Finder and Spotlight get environment variables from ~/.MacOSX/environment.plist (before 10.8) their own Info.plist (10.8) and launchd (system-wide) and not .tcshrc etc. Sounds like to get the updater to work from Finder for all users you have to set your $CCP

Re: [ccp4bb] off-topic: detergents for the stabilisation of water-soluble proteins

2012-10-13 Thread Bosch, Juergen
Going in a different direction with my reply here. Is your pI close to your current buffer ? Move at least one unit away from the theoretical pI. Do you have a friend with a real time pcr machine ? Then get some sypro orange and check out the thermal stability of your protein under various condit

Re: [ccp4bb] off-topic: detergents for the stabilisation of water-soluble proteins

2012-10-13 Thread Allan Pang
I also recommend you to check Dan's link on the paper for stabilising protein solubility. I have similar experience. I got high yield, soluble protein but aggregates once I have purified concentrated protein. Before venturing to the detergent area, I would suggest using other additives fi