They look like other phosphate crystals that I've seen, but have to shoot them
to tell.
From: CCP4 bulletin board [CCP4BB@JISCMAIL.AC.UK] On Behalf Of Nat Echols
[nathaniel.ech...@gmail.com]
Sent: 07 February 2013 22:30
To: CCP4BB@JISCMAIL.AC.UK
Subject:
Try
http://gentle.magnusmanske.de/
Resembles VectorNTI, is free, and works on Linux, Windows and Mac
(although I've only used it on XP).
Seems quite powerful, but not polished like the more commercial solutions.
Darren
On 08/02/13 05:16, Theresa Hsu wrote:
Dear all
Is there any good
On Feb 8, 2013, at 05:16 , Theresa Hsu wrote:
Dear all
Is there any good molecular cloning software for linux? It should read plasmid
constructs in Genbank format, do assembly of plasmid sequencing results,
display the sequencing trace and highlight regions where there are mutations
based on
Good morning Frank
On a related idea, do you typically use a limited number of buffers
(buffer plus salt) for the final purification step of your proteins?
If so, do you have a chart of where salt crystals may appear in the screens
that you use most often? Could you put that chart on your web
I didn't see the picture that you attached but if you have more than one
crystal you could always run one on a gel to see if it runs the expected size
of your protein
From: Patrick Shaw Stewart [mailto:patr...@douglas.co.uk]
Sent: Friday, February 08, 2013 11:47
Hi Patrick
Did you mean that to go to BB? To put pressure on us? :)
We've not done that analysis, no - good idea though. No standard
purification buffer, though most commonly it's HEPES pH 7.5ish, varying
amounts of NaCl and glycerol. Like most people, I assume.
There certainly are
There a lot of articles about salt-protein crystals in google - check
them first.
How to check crystals:
1) X-ray check (most obvious way)
2) izid dye
3)dry it (protein crystal will break apart)
4)crush it (if you dont know how to check by this method - try to grow
and break lysozyme or another
Patrick,
Something related:
http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Conditions_prone_to_salt_crystallization
Truth be told, we recently had a major breakthrough with the peg/fluoride
condition I came to consider a useless salt crystal generator. So tables like
these are
Like Nat points out, I suspect they are phosphate crystals. I have seen
those before.
And I agree 100% with Frank, for once. Why would I risk making any guesses
no matter how salt like my crystals look after all the time it took me to
clone, express, purify and crystallize my precious protein. It
Raji Edayathumangalam wrote:
(3) Inconclusive no diffraction situation, which could indicate a million
things including the possibility that your
cryoprotectant was sub-optimal for data collection done using flash
cryocooled/flash frozen crystals in a stream of
gaseous nitrogen.
But before
On Fri, 2013-02-08 at 09:13 -0500, Edward A. Berry wrote:
I like to take a 5-sec 180* oscillation which gives plenty of
spots in a nice pattern for a salt crystal
Second that
It also confuses bystanders really well - what a strange diffraction
pattern - half salt (small unit cell) / half
So, if you are bored and have nothing else to do (which is how we all
are at times; kidding), can you set up a control experiment with
everything in the crystal dip except protein (so buffer and whatever)?
I know protein plays a role in the process, but I have done this before
when I had
On Fri, 2013-02-08 at 14:53 +0400, Evgeny Osipov wrote:
Protein crystals behave rather as gelatine and not as solid
I'd have to disagree on that. Protein crystals are fragile but not
soft. If your crystals are like gelatine it's unusual. It has been
demonstrated that elastic properties of
I'd have to disagree on that. Protein crystals are fragile but not
soft. If your crystals are like gelatine it's unusual. It has been
demonstrated that elastic properties of protein crystals are similar to
organic solids
Interesting--do you have a reference quickly on hand for those
Journal of Crystal Growth 232 (2001) 498-501 and references therein
Available at
http://pages.physics.cornell.edu/~rthorne/publications/caylorjcg01.pdf
Colin
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jacob
Keller
Sent: 08 February 2013 14:57
To: ccp4bb
Subject: Re:
On Fri, 2013-02-08 at 09:57 -0500, Jacob Keller wrote:
do you have a reference quickly on hand
http://www.ncbi.nlm.nih.gov/pubmed/8129868
and references therein
http://www.sciencedirect.com/science/article/pii/S0022024801010922
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1300955/
The last
Ed,
Protein crystals are fragile but not soft.
If your crystals are like gelatine it's unusual.
I hate to disagree with the disagreement, but there are many exceptions to this
rule. I have seen many protein crystals that are quite malleable and bendable.
One protein produced rod-shaped
Michael,
It seems to me we have no disagreement, as we both say that it is
*unusual* for protein crystals to be non-fragile. Furthermore, my
objection is to gelatin characterization. I may be, as is my custom,
wrong, but in terms of elasticity gels are purely entropic. Protein
crystals, even
Am Mittwoch 06 Februar 2013 17:10:35 schrieb Yuri Pompeu:
Dear All,
I am trying to probe the existence of a disulfide bond on the surface of my
protein. I have attempted Ellman´s and my results were not as clear as I
would have hoped for. I am not a sulfur/cysteine chemist and would
Dear Colleagues,
Due to the application deadline originally scheduled for February 10,
overlapping with Summer holiday dates in several South American
countries, we have decided to extend the course application deadline
UNTIL FEBRUARY 17, 2013.
We invite all of you interested in this
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