Re: [ccp4bb] Difficult data

2013-04-17 Thread mjvdwoerd
Stephen, Stephen, Although your peptide is smaller than the one I once worked on, here are some thoughts that might be applicable. 1. Check and play with the radius of integration in the molecular replacement. The default is probably not appropriate for your case but the value should be much

Re: [ccp4bb] lcp crystal extraction

2013-04-17 Thread Konstantin Knoblich
Hi All, Just wanted to update this field in case someone in the future runs into the same problem as me. The problem was concerning the Laminex plates with the thick UV lid. The thickness of the lid cannot be stressed enough. While very good for preventing evaporation it was simply not practic

[ccp4bb] 3 Senior Structural Biology Faculty Positions Open

2013-04-17 Thread Mark A. White
University of Texas Medical Branch 3 Senior Faculty in Cryo Electron Microscopy, X-ray Crystallography, and NMR Sealy Center for Structural Biology & Molecular Biophysics UTMB Health seeks senior faculty applicants in structural biolo

[ccp4bb] Funded access to Automated Crystallization Services at EMBL Grenoble

2013-04-17 Thread Jose A. Marquez
-- -- Funded Access to Automated Crystallization Screening and in plate x-ray analysis a

Re: [ccp4bb] Difficult data

2013-04-17 Thread Herman . Schreuder
Dear Stephen, I guess you checked the processing that your huge unit cell is real. Did you try epmr? In this case an evolutionary search may be the most suitable method, but it could take a huge amount of cpu time. Some comments: Having about 40 monomers in the asymmetric unit could well explai