A couple of extra comments on top of Bob's rather comprehensive
recommendations, based purely on actually looking at Pilatus data (I
mean *looking*)
When you are inspecting the images looking at them at 100% size is
important: spots are small relative to pixels and the point spread is
essentially
Dear Fulvio,
what did your L-Test with the unmerged (P1) data look like? In order to
use twin refinement you should be sure that the structure is twinned.
Did you try all possible subgroups of R3? Generally, a triplet can be
treated as any other pseudo-merohedral twin, given it really is a
Graeme and Bob,
Wow... It's great to learn from actual experiences.
Thanks much for this write up.
If this were stackoverflow, +1.
F
On May 8, 2013, at 12:32 AM, Graeme Winter graeme.win...@gmail.com wrote:
A couple of extra comments on top of Bob's rather comprehensive
Dear pals,
Sorry for my off-topic question but its been a while I am challenging to get a
15 kDa protein coated on CM5 chip. Calculated PI of protein is 5.9 I tried
Acetate buffer pH5.2 and 3.8 for coating under normal procedure of Biacore.
After immobilization I saw this message: response
Hi Jahan,
Maybe you are using a new model of BiaCore. Our old BiaCore X never cares to
tell us how to do our experiment. However if your RU did go below that of the
empty chip, then maybe you really didn't get anything conjugated.
The pH 3.8 condition is not good for the amine coupling
Hi All,
I am trying to write a shell script to streamline a few steps, one of which
is Unique, see below. As you can see, this program requires symmetry and
cell parameters. In CCP4 GUI Scalepack2mtz, these info are automatically
extracted from .sca file (first two lines). But I don't know if
Hi Joe,
this line of code for csh/tcsh is awfully rickety-raggedy, it does the trick
though.
Jan
setenv spacegroup P3121
setenv noresidues 350
setenv lambda 1.5418
set unitcell = `head -3 ../hkl/output.sca | tail -1 | cut -b 4-60`
scalepack2mtz \
hklin ../hkl/output.sca \
hklout
