Applications are invited for a 3-year PhD position at Imperial College London
which will be based in The Membrane Protein Laboratory (MPL) at Diamond Light
Source, Didcot, Oxfordshire and the Centre for Structural Biology, Imperial
College London. The successful applicant, for the position which
Dear All,
I have been trying to scale (using SCALA) the diffraction data set collected
from multiple crystals. Data was indexed using mosflm but fails to scale with
an error saying
"negative scale factors" when scaling was tried in a constant mode whereas it
fails to scale with an error saying
Depending on the Space (Laue) group, there may be alternative indexing
possibilities which will be sorted out in Pointless - did you run that program?
I would recommend using the ccp4i task "Symmetry, Scale, Merge (Aimless)" which
uses Aimless instead of Scala - Aimless may be a bit more robust
Hi Rhys,
The bOG concentration of 1.0% is quite acceptable and if your protein is
stable and happy in OG, then you're in luck. At 30% PEG 600, I'm not
surprised you're seeing the phase separation or detergent 'blob's for lack
of a better term. I believe you have the latter, which many of us workin
Hello Rhys,
I suggest using an assay such as that described in Anal. Biochem.
336:117 to measure the amount detergent in your concentrated samples.
I found this assay accurate and easy to use with DDM. It should work
with b-OG too.
Good luck,
Ho
Ho Leung Ng
University of Hawaii at Manoa
As
*43**rd** Mid-Atlantic Macromolecular Crystallography Meeting*
*May 30 – June 1, 2013*
*Duke University*
*Durham, NC*
*ANNOUNCEMENT: *We have extended the deadline for submitting abstracts to
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and details can be fou
