[ccp4bb] Post-doc position for SAXS at the MAX IV Laboratory

2013-07-23 Thread Marjolein Thunnissen
The MAX IV laboratory in Lund has opened a position for a Postdoctoral fellow to strengthen the biological SAXS environment at the current SAXS beam line. The position will be for two years and is embedded in the BIOSTRUCT-X EU program. Full information of the position is available at: http://

Re: [ccp4bb] Where to cut the data in this medium resolution dataset

2013-07-23 Thread Kay Diederichs
Hi Stefan, you write "The diffraction pattern looks great, the 3.4A reflections are visible by eye and the edge of the detector is about 2.8A." and for the 3.4A data Mean((I)/sd(I)) in the highest shell is 2.3 . I'm tempted to ask: what prevents you from using higher resolution data to, say

[ccp4bb] Postdoctoral Research Associate at UCL

2013-07-23 Thread Katan-Muller, Matilda
Postdoctoral Research Associate-Structural Biology Applications are invited for a postdoctoral Research Associate position funded by Cancer Research UK programme in the field of signalling deregulation in cancer, covering structural and systems biology. The post holders will join an established

Re: [ccp4bb] Where to cut the data in this medium resolution dataset

2013-07-23 Thread Harry Powell
Hi I'd agree with Kay here - since the edge of the detector is at ~2.8Å. It is almost always worthwhile integrating to a higher resolution than you can see spots on the images - for what I would call "normal" datasets, I would always integrate to ~0.2Å higher (as a first estimate), then after e

[ccp4bb] IAEA- UPAT-GAEC Meeting: Second Research Coordination Meeting (RCM) on Utilization of Accelerator - Based Real - Time Methods in Investigation of Materials with High Technological Importance

2013-07-23 Thread Irene Margiolaki
ANNOUNCEMENT SECOND RESEARCH COORDINATION MEETING (RCM) ON UTILIZATION OF ACCELERATOR - BASED REAL - TIME METHODS IN INVESTIGATION OF MATERIALS WITH HIGH TECHNOLOGICAL IMPORTANCE   _24-27 SEPTEMBER 2013_   WEBSITE: http://iaeaupat.wordpress.com/ [1]   VENUE: Porto Rio hotel http:

Re: [ccp4bb] post to ccp4bb

2013-07-23 Thread Edward A. Berry
Engin Özkan wrote: On 7/22/13 11:20 PM, Ethan Merritt wrote: Which brings up the point that something seems to have gone wrong in one of your processing runs. Both runs claim mean (I/sigI) in the outer shell is 2.0, but in one case this is for the 2.4A shell and in the other case it's for the 2

[ccp4bb] 4th MX user Workshop at Diamond, 4th-5th September 2013

2013-07-23 Thread Thomas Sorensen
Dear All We will be hosting the 4th MX User Workshop at Diamond as part of the Synchrotron User Meeting, September 4th-5th, 2013. This year the workshop programme will cover new beamline capabilities, data processing and techniques. The meeting will also feature updates from the Membrane Protei

Re: [ccp4bb] post to ccp4bb

2013-07-23 Thread Engin Özkan
On 7/23/13 9:01 AM, Edward A. Berry wrote: Here we need some clarification on which I/s(I) is meant - sigma(I) for the individual measurements, or sigma(I) from error propagation to the final which as Engin notes will be lower. Isn't the reported Mean(I/sigI) in the reported Aimless table fo

Re: [ccp4bb] post to ccp4bb

2013-07-23 Thread Clemens Vonrhein
Hi, On Tue, Jul 23, 2013 at 10:11:04AM -0500, Engin Ozkan wrote: > Isn't the reported Mean(I/sigI) in the reported Aimless table for > the merged/averaged reflections (because that is what we are > discussing about)? Yes - according to 1. http://ccp4wiki.org/~ccp4wiki/wiki/index.php?title=Symme

Re: [ccp4bb] post to ccp4bb

2013-07-23 Thread Edward A. Berry
Engin Özkan wrote: Also, why would we be using the batch-wise I/sigma's for determining resolution cutoffs? Agreed. For cutoff I guess it should be the final sigma going into the refinement program that matters, irrespective of whether that was achieve by averaging more images or using a stro

Re: [ccp4bb] Where to cut the data in this medium resolution dataset

2013-07-23 Thread Stefan Gajewski
Nat, What do "correct" B-factors look like? What refinement strategy did you > use for them? > 1) If I see strong positive density in the Fo-Fc map along the backbone of two turns of an correctly placed alpha helix, therefore the B-factors are too high in that region. The model after refinement