[ccp4bb] EMBO Global Exchange Lecture Course in 2014- São Paulo, Brazil

Dear all, on behalf of Dr. Dmitri Svergun, I would like to draw your attention to the following EMBO Global funded course that we will host together with Prof. Cristiano Luis Pinto de Oliveria in **São Paulo** in January 2014. regards Margret

[ccp4bb] Job vacancy, University of York

VACANCY - (SENIOR) POSTDOCTORAL RESEARCHER IN GENE EXPRESSION FOR STRUCTURAL GLYCOBIOLOGY, University of York, UK We wish to appoint a postdoctoral researcher to work on the expression of mammalian genes, for 3-D structural and chemical mechanistic studies, in the area of eukaryotic glycobiology

[ccp4bb] Post-Doctoral position in Porto, Portugal - Deadline August 14th

Posted on behalf of Dr. A. Vale (avale_at_ibmc.up.pt), to whom all enquiries must be directed. The Fish Immunology and Vaccinology Group of Instituto de Biologia Molecular e Celular (Porto, Portugal) is seeking for a Post-Doc to work in the project “Studies towards identification of structural

Re: [ccp4bb] database of crystallization condition

Many thanks to all of you for useful links and suggestions. Best regards... Hena On Wed, Jul 24, 2013 at 7:51 PM, Bostjan Kobe b.k...@uq.edu.au wrote: Hena I agree with the responses so far, but I think It may not be a complete waste of time looking at the crystallization conditions for

Re: [ccp4bb] Google doodle for Rosalind Franklin

Thurs. July 25th, 2013 EBI also an interesting article on the subject of women in science then and today http://www.guardian.co.uk/technology/2013/jul/25/rosalind-franklin-google-doodle Miri Hirshberg, PDBe On Thu, 25 Jul 2013, David Schuller wrote:

Re: [ccp4bb] Maltose binding protein as a tag

Dear all, I would like to thank everyone for being so kind and sparing their time to respond. Just to summarise from all the responses that I got that may help someone else: MBP on its own is monomeric and tends to 'magically' help pretty much every protein you throw at it to become soluble. In

[ccp4bb] which dimer?

Hi all, I'm working with a protein that appears to be a dimer in solution, on SEC in runs as 24 kDa, while the actual mass of a dimer is 30. And I am trying to figure out which dimer is the biological one (it is a regulatory protein but details are uknown). The crystal structure gives me a

Re: [ccp4bb] which dimer?

Hi Karolina, You have an interesting case in hand but which is not uncommon. From your description it sounds as if option 2 is more likely to represent the biological dimer but as you noted, it's not certain. Best would be to obtain another crystal form (easily said, I know, not easily

Re: [ccp4bb] which dimer?

Hi Karolina, I would suggest you do SAXS. You can compare the scattering profile you get from your protein in solution to the calculated profiles of the three possible dimers (or other oligomers) you have in the crystal structure. This should give you a definitive answer. See for example this

[ccp4bb] fix atom during annealing in phenix.refine

Hi All, I am wondering if I can fix a set of selected atoms during simulated annealing in phenix.refine? I only found the option in xyz refinement, but not in annnealing. Thank you!

Re: [ccp4bb] fix atom during annealing in phenix.refine

Hi, I am wondering if I can fix a set of selected atoms during simulated annealing in phenix.refine? no. Pavel P.S.: There is Phenix mailing list for Phenix-specific questions