Dear Ansuman,
I agree with Matthew that the refinement seems to be OK - the reason the
bonds are longer is because what you actually have bound is MgF3- and
not AlF3. MgF3- is a much better transition state analogue as it is
isosteric and isoelectronic with a transferring phosphoryl group. At p
Dear Sreetama,
I would consider the possibility that this active site cysteine is involved in
a mixed-disulfide with beta-mercaptoethanol, which is present at a considerable
concentration in your protein buffer.
The fact that the residual density in both the Fo-Fc and 2Fo-Fc maps actually
incr
Hi Sreetama,
The water S-gamma distance made me think that it might be a cysteine
beta-mercaptoethanol adduct. Try building CME instead of CSO.
Cheers,
Robbie
-Oorspronkelijk bericht-
Van: "sreetama das"
Verzonden: 17-1-2015 19:27
Aan: "CCP4BB@JISCMAIL.AC.UK"
Onderwerp: [ccp4bb] ad
Dear all,
Can anyone tell me how to calculate number of frames from redundancy or
vica versa
Thank you
Maybe somehow do partial cys partial cme, refine occupancies—is this possible
in refmac?
JPK
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of sreetama
das
Sent: Saturday, January 17, 2015 3:11 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: Re: [ccp4bb] additional density on cysteine
I suspect it may be a reaction with your reducing agent. What did you use
either in the preparation, or in the crystallization. If you didn’t have
reducing agent it probably oxidized to sulfuric acid. You should figure it out
with difference maps and maybe mass spec also.
bob
> On Jan 17, 2015,
If there are no other noise sources, then then final signal to noise of
measuring the photons you describe is always EXACTLY the same. This is
why photon-counting is such a useful "error currency": doesn't matter
how you slice them up or lump them together. Photons are photons, and
the square
Dear all,
First of all sorry to put this off topic and silly question on bb. Can
anybody suggest me, how to create a stereo image and how it is different
from the normal. How can I visualize it, if anybody has answer for this
please suggest me its significance in analysis. Thank you very much in
a
