Dear CCP4bb,
I am facing a problem in generating .mtz output file from DETWIN program in
CCP4 (version 6.5). Program is finishing successfully with log file but not
mtz file.
Please suggest me a way out!
Thanks in advance,
--
Regards,
Sasha Pausch
Dear All,
I checked the Mg2+ B factor, and it was around 22. So, were the values for F
atoms. For AlF3, it was more than 50 for Al and more than 40 for F atoms. I
will take care about the occupancy. I hope that will fix the small negative
density.
When I inserted MgF in COOT using the code M
Hi all,
Actually, what I think has happened here is that Phaser has limited the
resolution to what it estimated was needed to get a clear enough solution, and
then the final refinement was carried out with all the data. The
signal-to-noise is lower with limited data, but it’s a price that seem
Hi Jeorge -
Something seems to have changed for the worse in this MR run. In your
earlier posting, where you failed to find a solution in P222, your log
file had solutions with RFZ=23.4, generally a clear indication of a
correct rotation function solution. (You didn't include this log file
The Z-values may be marginal, but of course you should inspect the
solution in Coot to see if the electron density makes sense, and the
packing of the protein molecules in the unit cell (look at symmetry
mates) is sensible. If this passes the sniff test, then you should clean
up your model (mak
Also, check the quality of the map going into density modification (by either
solomon or DM) and whether the output solvent mask is consistent with your
structure. Depending on the quality of the experimental phases, you can
generally see the outline of the molecule and see NCS in your experimen
Dear all,
A position has come available at Imperial for an X-ray facility manager
position. Responsibilities will include maintenance and training for our
local X-ray diffraction source and robotic crystallisation facilities, as
well as management of BAG time at the Diamond Light Source.
Please g
Hi everyone,
Many Thanks for your suggestions !! I checked for the everything related to
the components i am using : No contamination, not very old, no PEGs etc.
And then i followed the way Markov and Prem suggested !! For a trial set up
i just et up 10 different concentration variations of my pre
Hi Frank,
On Wed, Jan 28, 2015 at 07:52:39AM +, Frank von Delft wrote:
> Clemens - what do you mean by "correcting anisostropy"?
Ooops - typo and not quite clear ;-)
I mean diffraction anisotropy and correcting for it. SHARP will do
that by default if run through autoSHARP and if there is go
