Re: [ccp4bb] Shipping crystals for RT data collection

2016-12-23 Thread Jim Pflugrath
Ooops, I forgot to mention that oils would be another way to keep the crystal(s) on the loops. There are plenty of viscous sticky oils to choose from. They are some of the same ones used when flash-cooling crystals. -j On Fri, Dec 23, 2016 at 12:17 PM, Jim Pflugrath wrote: > A couple of ways

[ccp4bb] Shipping crystals for RT data collection

2016-12-23 Thread Jim Pflugrath
A couple of ways of shipping RT crystals: We would mount in capillaries, seal the ends with wax, then Duco cement or fingernail polish. Be aware that ambient air pressure will change alot during the shipping process, so the plugs could be compromised. I would take a box of pipetteman (or your fa

[ccp4bb] JOB: Field Service Engineer (Protein Crystallography) at Formulatrix

2016-12-23 Thread Dimitry Rodionov
Dear CCP4BB members, at first, this might seem like a completely misplaced posting about a job opportunity. But then, who could better understand the problems a crystallographer is having with his/her automation equipment than another crystallographer? The troubleshooting of imagers and liquid han

[ccp4bb] Citing the literature

2016-12-23 Thread David Schuller
I looked with distaste at the pile of notes I'd accumulated for my next Revere High Science Club lecture, on crystallography, which had been my specialty in graduate school and for many years after. The Carbon Murder, "A Gloria Lamerino Mystery" by Camille Minichino, 2004, ISBN 0-373-26522-0

Re: [ccp4bb] How can I refine B-factors only in CCP4 REFMAC?

2016-12-23 Thread Jon Agirre
Hi, you want to use these keywords: REFInement BONLY Good luck, Jon On 23 December 2016 at 06:52, Peng wrote: > > Dear all, > How can I refine B-factors only in CCP4 REFMAC? > I do not want to refine the XYZ coordinations. > Thanks > Peng > > > > > > -- Dr Jon Agirre York Structural Biol