Yes, CM-Sephadex expands and contracts incredibly. For other than batch methods
you will be much better using CM-Sepharose. I guess it is cross-linked
sepharose, and doesn't swell up much even in distilled water.
What is happening is the negative charges of the carboxy groups repel each other,
Hello All
I am using CM Sephadex C50 column for my protein. I equilibrated column with
MOPS buffer pH 6.0. But during elution I am using only NaCl2. This looks like
changing the bed height. Before starting elution the bed height was around
60cm. After starting elution the bed height decreased
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Presumably it counts all missing reflections in the refinement procedure.
Seems wrong!
What software are you using?
Eleanorr
Do an mtzdump to the resolution you used - that gives you a count of
observed reflections, plus the possible number of reflections..
On 7 February 2017 at 08:03, Vic Wu
Hi
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Dear All
when i deposit my structure, it show the "Number of unique reflections
measured(40854)"
but the "Number of Unique reflections refined against(48369)".
how could that happen?
I'd appreciate for any suggestion about this.
VicWu
