Re: [ccp4bb] How many reflections per shell for good statistics - data processing

2017-02-20 Thread Kay Diederichs
Hi Jorge, the answer probably depends to some extent on what you need the numbers for. The statistical error of the indicators is inversely proportional to the square root of the number of reflections. I seem to remember publications in the field of maximum likelihood refinement which report

Re: [ccp4bb] Lysozyme soaked with GlcNac?

2017-02-20 Thread PULSARSTRIAN
Dear Eike, You can refer to this article. The molecular basis of polysaccharide cleavage by lytic polysaccharide monooxygenases (2016 Feb) by Kristian, Glyn et al. Refer to the Supplementary Material of the paper for the information of soaking. (reference PDB id 5ACI).

[ccp4bb] Lysozyme soaked with GlcNac?

2017-02-20 Thread Schulz, Eike-Christian
Dear all, There are several structures in the PDB that show Lysozyme (mutants) in complex with GlcNac (or similar compounds). However, all of those structures seem to originate from co-crystallization experiments. I was wondering whether anybody knew a successful case of complex formation by

[ccp4bb] How many reflections per shell for good statistics - data processing

2017-02-20 Thread Jorge Iulek
Dear all,     Is there any study to recommend the (minimum) number of reflections for adequate statistics in each shell during image processing? I mean, for indexes like R_meas or R_symm or CC1/2, it might be important to know the number of total

[ccp4bb] X-ray Crystallography and Enzimology PhD scholarship at the University of Pavia

2017-02-20 Thread J Rubén Gómez Castellanos
The OXYTRAIN training network (see www.oxytrain.eu) offers 12 PhD fellowships for a program on mechanistic and applied enzymology research. OXYTRAIN is a joint academic/non-academic training initiative supporting the convergence of biochemistry, enzyme engineering and biotechnology. The most