[ccp4bb] CALL CCPFYP: Segmentation fault: 11 (macOS Sierra Version 10.12.4)

Dear all, I want to CALL CCPFYP in my fortran script, but after compile, the binary file can not be run (macOS Sierra Version 10.12.4). E.G. 1. : PROGRAM example_MTZ CALL CCPFYP stop end 2. gfortran-5 -O2 -o test test.f -L${CLIB} -lccp4f -lccp4c -lmmdb2 -lstdc++

Re: [ccp4bb] radiation damage-induced phasing (RIP) tutorial

That's more like a tutorial for XDS :P (thanks though) The problems: 1) It uses autorickshaw (which is a black box ...guess I'll read the paper today) ... and 2) my files were not recognized (MTZ with proper labels ...even the XDS_ASCII.HKL files ). Is it working? Guess I'll try to contact the

Re: [ccp4bb] CH-bond length discrepancies

Note, Molprobity has an option to use longer (neutron) X-H distances. Pavel On Fri, Apr 28, 2017 at 11:46 AM, Tristan Croll wrote: > I believe the reason for the discrepancy here is that MolProbity by > default places the hydrogens according to the centroid of the electron >

Re: [ccp4bb] CH-bond length discrepancies

I believe the reason for the discrepancy here is that MolProbity by default places the hydrogens according to the centroid of the electron cloud (most relevant to x-ray crystallographic data) rather than the nuclear position. In a polar bond like N-H the difference can be quite substantial.

[ccp4bb] CH-bond length discrepancies

Dear Fellows of the Bond, when validating a QM refined homology model with Molprobity, I noticed various 8 sigma deviations in the carbon-hydrogen bond distances. Out of curiosity, I then used refmac to calculate riding Hs for the same model, and at least in one instance (N-H backbone) there

Re: [ccp4bb] radiation damage-induced phasing (RIP) tutorial

There was a tutorial for MX including UV RIP available from the HZB in Berlin (BESSY MX group). Have a look at there website. I'm sure it is still available, or maybe they can send you the files on request. Cheers Christian Am 28.04.2017 um 17:12 schrieb Murpholino Peligro: Hi lads... Do

[ccp4bb] radiation damage-induced phasing (RIP) tutorial

Hi lads... Do you know if there is a good tutorial for doing RIP somewhere on the internet? What programs can do RIP? -SHELX -AutoRickShaw -? Thanks

[ccp4bb] SECOND ANNOUNCEMENT: Cryo-EM Symposium, 6-7 July 2017, European Photon & Neutron Campus, Grenoble, France

Dear all, This is the second announcement to the Cryo-Electron Microscopy Symposium that will take place from 6-7th July, 2017 in Grenoble, France. The aim of this symposium is to promote the exciting opportunities in structural biology opened by the advances in cryo-electron microscopy and

[ccp4bb] Postdoctoral Fellow Position at Rutgers University

Postdoctoral Fellow Position   Parashar laboratory at Rutgers School of Dental Medicine iscurrently seeking a highly motivated, creative individual with strong interestin the structure-function studies of proteins regulating bacterial signaling.   Qualifications: - Ph.D. in

Re: [ccp4bb] Sliconizing of cover-slips

Dear Praveen, I always siliconize my cover slips before using them for crystallization. Recipe is very simple. But make sure that you always use mask, gloves and perform your experiment under a fume hood. 1. 95% toluene + 5% dichloromethylsilane will give you a very good siliconization

[ccp4bb] Senior Research Scientist Protein Crystallography

To add additional expertise and capacity to our drug discovery service team in Munich-Martinsried, we are immediately looking for a Senior Research Scientist, PhD in Protein Crystallography. Protein Crystallographer As a Senior Research Scientist, you will support our team with

[ccp4bb] AW: [ccp4bb] Sliconizing of cover-slips

anyone tried rainex (if it's still on sale)? for us it works 1000x better than anything else: rugged, extremely water repellent, and cheap (one bottle lasts a lifetime). in the '90s at least you could get it in auto stores in the USA. cheers jon Von: CCP4 bulletin board

Re: [ccp4bb] Sliconizing of cover-slips

To me this begs the question: why do this at all? A cost issue? The Hampton coverslips work pretty well even with detergents that drastically lower surface tension like C8E4. Those of a UK competitor that i shall not name are not as good, in our humble experience. Bert

Re: [ccp4bb] Sliconizing of cover-slips

Ahh, this brings back memories of a former life, preparing hydrophobic coverslips for my surface chemistry experiments. I used chlorotrimethylsilane, and what I remember best is that the secret to a good coating is making sure your coverslips are utterly clean and dry. I used to clean them by

Re: [ccp4bb] Sliconizing of cover-slips

Hi Praveen, 20 years ago I seemed to spend more time siliconising cover slips than setting up the Magic 50. We had special jigs to hold a couple of dozen slips for washing, drying and finally immersion in silane solution. This method was very, very tedious. An alternative method was to pull a

Re: [ccp4bb] shadow at the edge

Abhishek The fun thing to do in these circumstances is to move the detector a known distance and see how much a point on the shadow expands (or contracts) from the centre of the detector. One can then ray trace (a simple diagram) to find the position of the object creating the shadow. In your

[ccp4bb] Post-doctoral research fellow positions available at Monash university

Dear All Post-doctoral research fellows: Lipid -mediated immunity MONASH UNIVERSITY Infection and Immunity Program, Biomedicine Discovery Institute Faculty of Medicine, Nursing and Health Sciences Prof. Jamie Rossjohn FAA FLSW, ARC Australian Laureate Fellow & Head, Infection and Immunity

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Provided your protein is >90 pure as per 15% SDS-PAGE analysis, you should try seeding. On 28-Apr-2017 12:43 PM, "李霞" wrote: Dear all: My protein is a demethylase,has failed to obtain protein crystals,then adopt the method of in situ enzyme and get protein crystals,but the

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Dear all: My protein is a demethylase,has failed to obtain protein crystals,then adopt the method of in situ enzyme and get protein crystals,but the crystal is still very very small,how to optimize can get diffraction crystal,please? Best X. L

Re: [ccp4bb] shadow at the edge

Dear Abishek, shadows should be excluded from processing since the outlier rejection algorithms cannot distinguish between weak and shadowed reflections! In XDS, you could use a generous static mask (UNTRUSTED_*), or a dynamic mask as specified by FILTER.HKL. HTH, Kay On Thu, 27 Apr 2017