Re: [ccp4bb] Rhodamine in FPLC

Dear Reza, I used hydrogen peroxide (~0.1-2%?) to remove rhodamine stuck on Superdex columns. "Pink" columns turned "white" after this kind of treatment. Hope that helps, Tomas On Fri, Jun 9, 2017 at 10:43 PM, Reza Khayat wrote: > Hi, > > Sorry for the non crystallography

[ccp4bb] Rhodamine in FPLC

Hi, Sorry for the non crystallography question. We're using Rhodamine for labeling some material that we need to run through our FPLC. It seems that the Rhodamine is getting stuck to the PEEK tubing. Anyone has experience with successfully removing the Rhodamine from the tubing? Thanks. Best

Re: [ccp4bb] preparing DL-Malic acid stock solution

Dear Janet, thanks for the excellent tip! It worked like charme! I think the reason why our solution turned milky was that we were adding NaOH pellets without controlling the temperature, and possibly its increase caused the (seemingly irreversible) transition to the “porridge” state. Your

[ccp4bb] Parsing error monomer library jLigand

Hi all, I installed the newest version of ccp4 package using the setup manager. When I launch jLigand from within Coot I get the error message "Cannot parse the standard monomer library". We checked if all paths and permissions are correctly set and couldn´t find any mistake. Also repeating

[ccp4bb] new version of XDS

Dear all, a new version for academic users of XDS has been posted. The major changes are: - a CLUSTER_NODES keyword that lets you use several machines in a flexible and easy way - (bug fix) Eiger data can (again) be read, preferentially using Dectris' Neggia library - new RELRAD keyword that