Have you looked for other possible models in the PDB?
Eleanor
On 21 July 2017 at 02:16, 张士军 <21620150150...@stu.xmu.edu.cn> wrote:
> Hi everyone
>
>I have got a anti-parallel coiled-coil structure in a short fragment
> recently, then I want to solve a longer fragment structure with phenix-MR
James,
On
On 20 Jul 2017, at 19:06, James Holton
mailto:jmhol...@slac.stanford.edu>> wrote:
In my experience you need at least an average of 1 photon/pixel/image before
even the best data processing algorithms start to fall over.
I do not agree… both XDS and DIALS will (today) to pretty well
Hi everyone
I have got a anti-parallel coiled-coil structure in a short fragment
recently, then I want to solve a longer fragment structure with phenix-MR using
this short fragment structure as a model.The result is not good because of the
Rwork and Rfree is high.So I think the longer fragme
Hello Mac Gurus,
I have run today into a problem with XQuartz on my iMac. No problems till now.
Now, any application that uses X11 does not start. It invokes XQuartz but that
is it. What is more, when running, XQuartz can not be stopped; after an attempt
to quit the application XQuartz immediate
I’m intrigued by the prospect of using AMPLE to test multiple distant homologs
in a MR problem. I’ve used HHPRED to identify about 20 high-probability
homologs of known structure, each of which has about 20-25% identity with the
unknown protein. However, it’s not clear to me from the documentati
Based on this, a vision for the future:
A warehouse filled with sealed-tube, top-hat-profiled sources, super-accurate
goniostats, and Dectris detectors, a robot running back and forth from a
central dewar to place the crystals, all images 1-bit, intensities measured as
probabilities; a day when
An important aspect of fine phi slicing that has not been mentioned yet
(and took me a long time to figure out) is the impact of read-out time.
Traditionally, read-out time is simply a delay that makes the overall
data collection take longer, but with so-called "shutterless" data
collection th
Dear Gerard and Ian,
thank you for your work in putting together the Staraniso sever and your
effort to tackle the anisotropic diffraction problem, a non-trivial
behavior that creates many problems in structure solving, and that is
very prevalent in the world of membrane proteins...
I have b
please characterize your a and b to see they really what they are
发自网易邮箱大师
在2017年07月18日 10:25，高艺娜 写道：
Hi all ,
It has been reported the Negative stain EM of a protein A-B complex, but
according to my gel filtration results (I purified A and B respectively for
incubation) , I found that A co
