Thank you Eleanor,
I did the PISA analysis and one of the "high B" chains in the region of my
metal had a BSA ~ 10% lower than that of the "Low B" chain. I don't know if
that is a significant difference.
Best
Denis
From: Eleanor Dodson [eleanor.dod...@yo
Dear All,
I am posting the following information on behalf of Professor Hong Li, IMB,
Florida State University, Tallahassee, FL. All inquiries should be sent
directly to her. Thanks.
An NIH-funded postdoctoral position is immediately available in Dr. Hong Li's
laboratory at the Institute of
I would submit the coordinates to PISA to analyse the buried surface area
for each momnomer and see if there are differences. It is pretty common
that some copies have much lower B values and therefore are better
defined..
Eleanor
On 29 November 2017 at 17:15, Denis Rousseau wrote:
> I want to
I want to thank everyone on the BB for their comments about the differences in
the homodimeric protein. I had done a superposition of the structures and found
no differences that could account for the differences in the water occupancy I
observed. However, I had not compared the B factors which
I have seen this in MnSOD. We had a tetramer in the ASU and each
active site had different ligands in our peroxide soak. I assumed the
crystal lattice can influence these things or it is inappropriate
metal incorporation. I would highly recommend doing ICP-MS on a
dissolved crystal and on your pu
Research Fellow in Structural and Computational Biology
Many of the proteins that are critical for cancer development are intrinsically
disordered or have large disordered regions. Moreover, the genetic alterations
that promote cancer generate mutated forms of proteins that are destabilized.
On
Thank you everyone for your contributions that help me understand more.
Let me try to rephrase my initial post:
Say that I have diffraction up to 6A resolution, I would say that my
crystal is decently ordered (see the glass half full), enough for
observing repeating units that do not fluctuate
Denis,
Others may have addressed this, but you have not mentioned your refinement
conditions. At 2.4 Å, are you using what and how early in the refinement is
your work? NCS restraints, group B refinement, individual B refinement, etc.
A little more information would help the BB readers respo
That depends on the quality of your data and your model!
I play with refinement and rebuilding - check indicators v resolution., etc
etc (I look at the v plot from REFMAC - can show up problems
with low resolution data.
Eleanor
On 29 November 2017 at 12:46, YUVARAJ I wrote:
> Dear all
>
Dear all
Iodine gave a good anomalous signal at copper k-alpha
wavelength. Thank you everyone for your good suggestions. I could build the
model and refine it and bring the R-factor/ R-free 0.214/ 0.247 at the
resolution of 1.6A (with the previous data set without anomalous signal,
reso
A PhD position is available in membrane protein biochemistry and X-ray
crystallography in the research group of Dr. Bjorn P Pedersen at the
Department of Molecular Biology and Genetics, Aarhus University, Denmark.
Our group studies the structure-function relationship of membrane proteins
involved
Hello
I have two questions relating to the same protein build. I am struggling with
an interesting case of a protein structure for which we have chosen to build it
with microheterogeneity. In summary, we have an all alpha helical highly
repetitive solenoidal RNA binding protein. In our structur
Dear all,
This is a reminder that there is an open position for a specialist in
biological X-ray techniques (especially SAXS plus X-ray diffraction and
crystallisation) at the Centre of Molecular Structure (a CIISB and Instruct
site) in Vestec, Prague region. The deadline for submission of appl
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