The SAUC Cell Database has been updated to 122596 PDB cells and 397452 COD
cells. SAUC is accessible at
http://iterate.sourceforge.net/sauc
or
http://flops.arcib.org:8084/sauc/
The second link is to a faster machine, but with less network bandwidth
If you would like to install your
All true, I did my undergraduate thesis (at cambridge about 10**6 years ago it
feels) on the formation of carboxylate-pairs in the gas phase…. But I agree
with the thread: there is a metal there; it’s almost certainly not Mg, but it’s
hard to say without further data whether its Mn, Fe, Cu, Zn
Hi Yuvraj,
Here are some thoughts in addition to what's been suggested already:
1. A good recent reference is here:
http://journals.iucr.org/f/issues/2015/06/00/en5564/index.html
2. The image you sent suggests cubic ice, indicating a higher glycerol
concentration (maybe 35 or 40%) is needed.
Dear colleague,
Registration is open for the Les Houches/TSRC Workshop on Protein
Dynamics, to be held in Les Houches, close to Chamonix at the heart of
the French Alps, from May 27 to June 1, 2018.
This international workshop is a forum for presenting and discussing
results from
In this case, yes - there’s clearly a metal ion coordinated there. But for the
record, it is entirely possible (if somewhat rare) to get acidic side chains
directly contacting each other. Look up “carboxyl-carboxylate pair”. They’re
typically found at low pH and/or in quite protected
Hi Yuvaraj,
the importance of proper cryo mounting is under-appreciated in the community.
Intensities of reflections on or close to ice rings cannot be recovered by the
data processing software with good accuracy, which is detrimental for structure
solution and refinement. Thus it is vital to
Hi, Yuvraj,
I can suggest two methods:
1) collect a dataset at room temperature
2) crystallize your protein in cryoconditions. That is add 25 % of
glycerol to your crystallization conditions and perform small optimization
in terms of precipitant concentration.
2017-12-29 7:55 GMT+03:00 Prem
