Re: [ccp4bb] Dose in diffraction patterns?

2020-05-06 Thread James Holton
In general?  No. I believe a few places put "flux" into the header, but as Andreas just mentioned that is only one of the bits of information you need to calculate dose.  If all you want is a rough estimate, then the numbers you need are: f = flux (photons/s) t = exposure time (s) w =

Re: [ccp4bb] CCP4 source code

2020-05-06 Thread Fischmann, Thierry
Thank you for making the 7.1 source available ! For those interested, http://www.ccp4.ac.uk/download/index.php#os=src Thierry From: CCP4 bulletin board On Behalf Of Fischmann, Thierry Sent: Monday, May 4, 2020 3:26 PM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] CCP4 source code EXTERNAL

[ccp4bb] PhD Position in Structural Biology

2020-05-06 Thread Roman Fedorov
The research group headed by Prof. Dr. Dietmar Manstein invites applications for a PhD position funded by the Health Research Framework Programme of the German Federal Government and the European Joint Programme on Rare Diseases under the call "Transnational Research to Accelerate the Diagnosis

Re: [ccp4bb] disinfecting keyboards

2020-05-06 Thread Jurgen Bosch
Must have been wearing a traditional Scottish outfit :-) Jürgen > On May 6, 2020, at 11:19 AM, Tristan Croll wrote: > > You got off lucky. An old friend of mine learned this lesson when on a > particularly sunny day he spent an hour out on a New Zealand glacier in > shorts with no

Re: [ccp4bb] disinfecting keyboards

2020-05-06 Thread Tristan Croll
You got off lucky. An old friend of mine learned this lesson when on a particularly sunny day he spent an hour out on a New Zealand glacier in shorts with no underwear... On 2020-05-06 16:17, James Holton wrote: I feel I should correct you on one thing Tim: UV _can_ go around corners because

Re: [ccp4bb] disinfecting keyboards

2020-05-06 Thread James Holton
I feel I should correct you on one thing Tim: UV _can_ go around corners because it scatters.  I learned this the hard way as a younger man after a fine day of skiing.  I had put sunscreen everywhere except the bottom of my nose. You are right, however, that the intensity after scattering is

[ccp4bb] IMCA-CAT beam time is available for proprietary experiments

2020-05-06 Thread Jesse Yoder
Dear Colleagues, The IMCA-CAT beamline at the Advanced Photon Source (APS) is continuing to operate and collect crystallography data during the current restricted-access operations at Argonne National Laboratory. Experiments are limited to (1) critical research related to COVID-19 or (2)

[ccp4bb] PDBe-KB COVID-19 pages: more download options and antibody annotations

2020-05-06 Thread John Berrisford
We recently announced the new PDBe-KB COVID-19 data portal (pdbe.org/covid19), allowing users to easily access a wealth of structural data from the SARS-CoV-2 virus. We have now added annotations on antibody classifications and details of peptide inhibitor molecules to the interactions section. In

[ccp4bb] Structural Biology- researcher position available

2020-05-06 Thread Elżbieta Nowak
Dear All, posted on behalf of Dr. Marcin Nowotny, IIMCB Laboratory of Protein Structure at the International Institute of Molecular and Cell Biology in Warsaw (http://bit.ly/nowotny-lab) seeks a researcher (full time position) to work in a large multinational consortium project that aims at

Re: [ccp4bb] Fusion proteins to solubilize your protein

2020-05-06 Thread Jose Artur Brito
Dear Murpholino, a portuguese company called NZYtech has a collection of fusion tag pHTP expression vectors (https://www.nzytech.com/wp-content/uploads/2019/04/nzyeasy-cloning-expression-system_userguide_v1901.pdf), some of them designed for increased protein's solubilization. We have a

[ccp4bb] Structural Biology-researcher position available

2020-05-06 Thread Elżbieta Nowak
Dear All, posted on behalf of Dr. Marcin Nowotny, IIMCB Laboratory of Protein Structure at the International Institute of Molecular and Cell Biology in Warsaw (http://bit.ly/nowotny-lab) seeks a researcher (full time position) to work in a large multinational consortium project that aims at

Re: [ccp4bb] disinfecting keyboards

2020-05-06 Thread Tim Gruene
Hi James, for us, the suggestions of cling film / plastic wrap or just swapping keyboards and mice per person is the simplest - thanks to everyone for the many suggestions. Especially the latter, since only two people will operate the instruments. UV light does not go around corners. It might be

Re: [ccp4bb] Dose in diffraction patterns?

2020-05-06 Thread Andreas Förster
Dear Murpholino, all diffraction patterns contain information on absorbed dose in the form of radiation damage. You might be more interested in a quantitative description of dose. For this, you need information on the size and shape of your crystal; the sequence of your protein + cofactors,