[ccp4bb] tetrameric tag for extracellular proteins

Hi all, Following what someone recently suggested, Fc tag is a great way to dimerize a secreted protein. I have successfully tried the TNF receptor-associated factor 1 tail to *trimerize *and the COMP (cartilage oligomeric matrix protein) to *pentamerize *my monomer but I can't identify a suitable

Re: [ccp4bb] on space group P 3 2 1

Hello, all you ever need is: https://www.ebi.ac.uk/pdbe/pisa/ Best wishes, Jon Cooper. jon.b.coo...@protonmail.com Original Message On 25 Sep 2020, 14:35, Smith Lee wrote: > Dear All, > > For pdb ID 1g4a, it is of space group P 3 2 1. In the paper for this pdb > (Crystal Stru

Re: [ccp4bb] on space group P 3 2 1

Dear Lee, I would recommend that you visit: https://www.ebi.ac.uk/pdbe/entry/pdb/1g4a/analysis there you can download the assembly indicated by the REMARK 350 Octadecamer that describes the "AUTHOR DETERMINED BIOLOGICAL UNIT". The download will be in mmcif format that many programs can support

Re: [ccp4bb] on space group P 3 2 1

:: which indicates the 6 molecules are far away from each other. Those are just the symmetry operators for space group P321. It tells you nothing about how far apart the molecules are, although the cell dimensions do in fact impose some constraints. However look at REMARK 350 in the PDB format

[ccp4bb] on space group P 3 2 1

Dear All, For pdb ID 1g4a, it is of space group P 3 2 1. In the paper for this pdb (Crystal Structures of the HslVU Peptidase–ATPase Complex Reveal an ATP-Dependent Proteolysis Mechanism), it has been configured to compact hexamer from the pdb 1g4a, with the pdb 1g4a can be regarded from a dimer

[ccp4bb] Postdoc positions at MRC-LMB & CCPEM/STFC

Dear all, For those interesting in computational cryoEM development... We are excited to announce two new PDRA positions in the Scheres’ group (MRC-LMB, UK) and the CCP-EM group (STFC RAL, UK). These two positions will work closely together as part of a wider project with the Alan Turing Insti

Re: [ccp4bb] dimeric tag to induce the homodimerization of protein

Dear Dhiraj, You can use coiled-coil domain to dimerize your protein. Please look at those exemplary publications: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3923517/#R12 https://www.nature.com/articles/nsb877 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3375751/ https://pubs.rsc.org/en/conten