Re: [ccp4bb] N-terminal PCA as artifact of crystallization?

Thank you for all the references and comments (as always). I understand now that in the context of the protein N-terminus, cyclization of Glu to PCA appears to happen spontaneously at low pH and therefore is also possible with non-eukaryotically expressed samples. I'll look more closely at the va

[ccp4bb] Future Planning: Entries with extended PDB and CCD ID codes will be distributed in PDBx/mmCIF format only

http://www.wwpdb.org/news/news?year=2021#607760112786e73a79c76f9d wwPDB, in collaboration with the PDBx/mmCIF Working Group , has set plans to extend the length of ID codes for PDB and Chemical Component Dictionary (CCD) ID entries in the future. Entries issued

Re: [ccp4bb] N-terminal PCA as artifact of crystallization?

Hello, there's another one at 1.1 A in 5JK4 which was a contaminant that crystallised over 2 years. No mass-spec, though! Cheers, Jon.C. http://scripts.iucr.org/cgi-bin/paper?S2059798316010433 Sent from ProtonMail mobile Original Message On 21 Apr 2021, 17:01, Isabel Moraes wr

Re: [ccp4bb] N-terminal PCA as artifact of crystallization?

Dear Jared, I advise you to have a look into our very recent Nat Comms paper (in particular supplementary information) https://doi.org/10.1038/s41467-020-20596-0 In our high-resolution crystal structures of the light-adapted (6S6C) and dark-adapted (6GUX) state of Archaerhodopsin-3 (AR3), so

[ccp4bb] Job posting: Scientist-I (Structural biology) at Frederick National Laboratory for Cancer Research

Hello everyone, The Frederick National Laboratory for Cancer Research is looking for a Scientist with experience in protein chemistry and structural biology (crystallography). The candidate will join the structural biology group within the NCI RAS Initiative program and work on the protein-protein

[ccp4bb] N-terminal PCA as artifact of crystallization?

Dear all, I'm looking at a crystal structure (1H4G) where the N-terminal Glu residue has cyclized to pyroglutamic acid (PCA). The protein was expressed in and secreted from bacteria (*Bacillus licheniformis*), and the crystallization conditions for 3 ul hanging drops were 2 ul protein solution (1

[ccp4bb] CASE PhD project available on DNA helicases - University of York and Oxford Nanopore Technologies.

Dear All Can I request your assistance in finding a PhD student? We have a 4-year fully-funded CASE PhD project available for an October 2021 start. The project is a collaboration between myself, Professor James Chong (York) and Oxford Nanopore Technologies. The project will include structural

Re: [ccp4bb] ccp4i2 crashes on MacOSX 10.13.6 - High Sierra

Blimey, I’m surprised I found this about 15 minutes after posting, having spent all this week trying to locate it. It *seems* that I had libs from an openssl installed via Homebrew that the openssl supplied with CCP4 found before its own. Deleting those files (/usr/local/lib/libssl.1.1.dylib an

[ccp4bb] ccp4i2 crashes on MacOSX 10.13.6 - High Sierra

Hi folks I have an old-ish Mac (Mac Pro, mid-2010, 3.46 6-core Intel Xeon, 32GB RAM) running High Sierra (OSX 10.13.6) - the box will not support newer versions of OSX or MacOS, so I can’t “upgrade” any further on the OS front (well, I could if I really wanted to hack beyond Apple’s recommendat