RESEARCH / POSTDOC ASSOCIATE
An NIH-funded research position is available in the Tsai laboratory at Baylor
College of Medicine to determine the 3D structure, mechanism, and function of
ATP-dependent protein quality control machines and related stress-response
proteins using protein crystallogra
Hi Rohit
Since you are saying the C terminus in crystal structure is not
end of your protein in crystal, and you are worried about its charge status, is
in't a good idea to model few residues beyond the C terminus you see in crystal
structure using weak electron density and crystal
You could replace the OXT with a hydrogen, note O-H bond length is about 1
Angstrom, a la neutron style refinement. Phenix has this sort of thing inbuilt,
I think.
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Original Message
On 23 Sep 2021, 10:06, Schreuder, Herman /DE wrote:
> Dear Rohit,
>
https://www.epfl.ch/about/working/professor-in-physics-of-imaging-ecole-polytechnique-federale-de-lausanne-epfl-and-head-of-mathematics-and-physics-of-imaging-paul-scherrer-institute-psi/
Prof. Gebhard F.X. Schertler
Structural Biology ETH Zürich D-BIOL
Head of Biology and Chemistry
Division
Dear Rohit,
Do you mean that the residues after your "C-terminus" have been deleted because
there is no convincing electron density for them?
In that case, a charged carboxylate at the N-terminus is incorrect and you
should delete the OXT atom. Alternatively, you could add one residue at the
C-
