Ahh, waters. Where would structure-related debate be without them?
I see. So if your default refinement procedure is to add an unspecified
number of waters, then yes Rwork might not be all that useful, as it
will depend on how the building goes.
Again, it all depends on what you want your da
"PDBLOCAL " should do exactly what you want:
https://www.ccp4.ac.uk/html/mrbump.html#pdblocal-directory
Dr Richard Gildea
Data Analysis Scientist
Tel: +441235 77 8078
Diamond Light Source Ltd.
Diamond House
Harwell Science & Innovation Campus
Didcot
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Hi Clemens
OK so you're saying use only the reflections that are in common between all
datasets and keep the parameterisation the same. There are clearly two
distinctly different ways in which datasets can differ: either a different
set of indices due to different resolution cut-offs or completen
On Wed, Nov 03, 2021 at 12:54:00PM +, Ian Tickle wrote:
> Suppose you had to compare two datasets differing only in their
> high-resolution cut-offs. Now Rwork, Rfree and Rall will inevitably have
> higher values at the high d* end, which means that if you apply a cut-off
> at the high d* end
Dear Community,
I just came accross a paper on the use of MrBUMP to fit pdb models to cryo-EM
maps: https://scripts.iucr.org/cgi-bin/paper?S2059798321009165
However, it turned out that the program tries to download coordinates directly
from the pdb, which our firewall does not allow. Is there a
A two-year PostDoc position according to German pay grade 13, with the
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‘Structural biology of membrane repair’, with a focus on cryoEM studies.
The Daumke
Please pass on to students/colleagues who may be interested.
The Department of Biological Sciences at Birkbeck, University of London
is offering an on line, postgraduate course module on Macromolecular and
Cellular Electron Microscopy, in the spring term January-March 2022. It
will cover the b
Dear CCP4 community,
I would like to share our new software for data analysis of Molecular
Biophysics experiments The platform enables users to upload and analyse
raw data from several experimental techniques: steady-state fluorescence
spectroscopy, microscale thermophoresis, and differential
