Re: [ccp4bb] Strategies for increasing Trp content of proteins

We have had success placing single trp on the outside of proteins, as weird as it sounds it works pretty well. If your protein is easy to express you can try a few choices. If your protein is difficult to express, a fusion with a trp-positive domain or with gfp may be a better option. There are m

[ccp4bb] Strategies for increasing Trp content of proteins

Dear CCP4BB, We are working on a protein that has no Trp residues, which makes chromatography challenging due to the low absorbance at 280 nm (Abs 0.1% = 0.104). Does anyone have experience using mutagenesis to increase the Trp content of proteins? Thanks, Jack Tanner -- John J. Tanner Profe

[ccp4bb] 4-year PhD position in Bergen, Norway

Hi, we have an opening for a fully funded 4-year PhD position in my group at the Unviersity of Bergen, Norway. The position is funded by a recent grant from the Norwegian Research Council, and the broad topic is the assembly and properties of multilayered proteolipid membranes. Please see the

[ccp4bb] PDRA in software development at York...

Hi all We have a position available at York to develop web based tools for molecular graphics and model building. For details, see this page . -- Professor Kevin Cowtan Email: kevin.cow...@york.ac.

[ccp4bb] CCP-EM Spring Symposium VIII 2022 3-5th May 2022

Dear CCP-EM, CCP-EM are pleased to announce the eighth annual 'CCP-EM Spring Symposium', 3-5th May 2022. The conference aims to provide a forum to highlight state of the art developments in computational cryoEM and related themes as well as showcasing outstanding recent biological applications

[ccp4bb] Boehringer Ingelheim is looking for new talents!

Boehringer Ingelheim is currently looking for a "Post Doc Integral Membrane Protein (Structural Biology)" (Ref. 220769) located in Biberach (Riß), our biggest R&D site in Germany. You can find the most important information below: THE POSITION Take your chance to work with a state-of-the-art-stru

[ccp4bb] PDBe updated mmCIF files now available via FTP

Dear Colleagues, PDBe has madePDBx/mmCIF files, containing SIFTS mapping information and additional, standardised metadata available directly from the EMBL-EBI FTP area, from: https://ftp.ebi.ac.uk/pub/databases/msd/updated_mmcif/ PDBe’s release process creates these enriched PDBx/mmCIF files

[ccp4bb] post-doc and PhD position at KU Leuven, ligand-gated ion channels

This is a kind reminder that the positions of post-doc and PhD in our lab are still open for applicants. Best regards Chris > On 18 Jan 2022, at 16:20, Chris Ulens wrote: > > Dear members of the community, > One post-doctoral position and one PhD position are available in the > Laboratory of