[ccp4bb] Funded PhD position in Newcastle University (CRISPR tagging, protein complexes, cryo-EM)

2023-01-25 Thread Wyatt W. Yue
Dear all, I would be grateful if you would please circulate the below PhD opportunity to potential candidates in your network. Many thanks. Wyatt Yue Professor of Structural Biology Biosciences Institute Newcastle University NE2 4HH Profile: wyattyue.github.io *CRISPR-mediated tagging of

[ccp4bb] Beamline scientist job opening at NE-CAT at the APS

2023-01-25 Thread Salbego, Cyndi
The Northeastern Collaborative Access Team (NE-CAT), located at the Advanced Photon Source at the Argonne National Laboratory in suburban Chicago, is seeking applicants for a Staff Scientist position at the Research Associate and Sr. Research Associate levels. Primary responsibilities will be

Re: [ccp4bb] Coot issue

2023-01-25 Thread Nicholas Clark
Mirek, Yes, this is a known issue (I read about it previously either here or on the Coot webpage, I can't recall which). The only "workaround" is to check the "keep delete active" box before you begin deleting. It took me a bit to realize this, so I'll share: The window is still open and can be

[ccp4bb] Coot issue

2023-01-25 Thread Cygler, Miroslaw
Hi, I just upgrade my Mac to Ventura v13.2 from v12.6.3 and run into issue with Coot. When I use the delete command it causes the main Coot window disappearing while other Coot windows stay open. Has anyone had this issue? Is there a way around it? No such problem on v12.6.3. Best, Mirek

Re: [ccp4bb] Map superposition!

2023-01-25 Thread Pietro Roversi
Thank you Pavel, very useful. I imagine I have to invert the hand *beforehand* if it is not the correct one :-) Just saying - checking hand and picking the better one would be a nice feature to implement in this tool. P On 25-01-2023 17:06, Pavel Afonine wrote: Hi Rams,

Re: [ccp4bb] Map superposition!

2023-01-25 Thread Pavel Afonine
Hi Rams, phenix.match_maps will superpose maps and models. Totally general: target/moving boxes (unit cells) and symmetry can be different, gridding can be different too. I can help off list should you need any assistance! Pavel On Wed, Jan 25, 2023 at 6:30 AM Subramanian, Ramaswamy wrote:

Re: [ccp4bb] Map superposition!

2023-01-25 Thread Bruno KLAHOLZ
Hi Rams, did you try to pre-align one map on the other by first moving it manually in chimera (X) and then only use the "fit in" option for automatic alignment? Automatic alignments often don't work from scratch, but if you try different manual prealignments it helps getting out of a local

[ccp4bb] Map superposition!

2023-01-25 Thread Subramanian, Ramaswamy
Hi All, I have two maps - both cryo-EM. One with and one without ligand. I am trying to superpose them and I cannot seem to get ChimeraX to work. I then tried VESPER and that also does not work. The built models superpose very well. What is the best way to superpose maps. Thank you.

[ccp4bb] Juan de la Cierva candidacy - 2 years postdoctoral contract - Plastic and mitochondria

2023-01-25 Thread Maria Sola i Vilarrubias
Dear CCP4ers, Our group is looking for a highly motivated postdoc with competitive CVs to apply for a 'Juan de la Cierva FormaciĆ³n' contract by the Spanish Ministry of Science and Innovation. The PhD thesis of the candidate must have been defended between *January* *1st 2021 and December 31st

[ccp4bb] Postdoctoral position to characterise novel plastic degrading enzymes

2023-01-25 Thread Marko Hyvonen
Dear Colleagues. Still six days to apply for an exciting opening in our group for a postdoc to work on characterisation of new plastic degrading enzymes ("plastizymes") in a BBSRC-funded sLoLa project lead by Florian Hollfelder and in collaboration with Christine Orengo (UCL) and Rob Finn

[ccp4bb] Rockimager (spares) looking for a new home

2023-01-25 Thread Marko Hyvonen
Hello all, We are in the process of replacing our Formulatrix Rockimager system and getting rid of the old one. Don't have the exact specs at hand, but it is >10 years old system with 1000 plate capacity and with both visible and UV imaging. It is in full working order, but beyond support by