Re: [ccp4bb] What could these crystals be?

I had the same issue before. PAS should be a decent polymer for modification. On Thu, Nov 9, 2023 at 7:04 PM sadik sattar wrote: > Hi Careina, > > I hope this email finds you well. I wanted to share my experience with a > similar issue I faced in the past concerning one of my proteins, and I've

Re: [ccp4bb] What could these crystals be?

Hi Careina, I hope this email finds you well. I wanted to share my experience with a similar issue I faced in the past concerning one of my proteins, and I've attached a picture of my round crystals. Despite numerous attempts with various screens and additives, I consistently encountered these

Re: [ccp4bb] [ccpem] Making thioester bond in Chimera/ChimeraX

Hello Firdous, Probably the problem is that mol A and mol B are two different models. You can't add a bond in Chimera or ChimeraX until after combining them into one model first. ChimeraX combine command: ChimeraX bond command or

[ccp4bb] Video link for RSC meeting on "British X-ray Crystallographers."

Message from the conference organiser: Dear all, I am very glad to be able to tell you that all the talks given on 18th October have been put up on the RSC Historical Group YouTube playlist: [https://www.youtube.com/playlist?list=PLLnAFJxOjzZu7N0f5-nVtHcLNxU2tKmpC](https://outlook.office.com/ow

Re: [ccp4bb] What could these crystals be?

Dear Careina, I usually see such kind of "Quasi-crystal" when the folding force was not properly adjusted. Of course, you need to verify they are protein ppt before any further modifications. For these melon-seed-like crystals, the sharp-end indicated that some crystallization occurred. However,

[ccp4bb] Making thioester bond in Chimera/ChimeraX

Hi Does anybody know how to make thioester bonds in Chimera or ChimeraX ? I am unable to make a bond between Cys321 in mol A with Gly1 in mol B (S-glycyl-L-cysteine) using Chimera or ChimeraX. I tried Isolde in ChimeraX and Build Structure in Chimera to make a bond between selected atoms but so

Re: [ccp4bb] What could these crystals be?

It’s easy to do on a 0.2 µm spin filter, just pipet the crystals onto the filter with excess mother liquor, wash with several spins of mother-liquor, and elute with SDS loading buffer. Run all the washes and elution on a gel. -- Kevin M. Jude, PhD Structural Biology Research Specialist, Garcia L

[ccp4bb] problem in sugar refinement with CCPEM

Dear All, I am trying to refine a cryoEM structure containing a simple glucose disaccharide using CCPEM + CCP4-8.0 but I get the following error from Refmac Servalcat : ERROR : atom :O1   BGC 1  Z4_  is absent in the library ATTENTION: atom:O1   BGC 2  Z5_  is

[ccp4bb] CryoeM RA post Newcastle

Dear All, I am looking for enthusiastic specialists in CryoEM (experience from grid preparation to model building) to study molecular mechanisms of transcription – various complexes of RNA polymerase with nucleic acids, different factors and fellow cellular machineries. There is a number of pr

[ccp4bb] Six year Post-doc at the MX beamline (XALOC) at the ALBA synchrotron, deadline 15/11

Dear community, We have an opening for a post-doc at the MX beamline XALOC for a total duration of up to six years. We are looking for a highly motivated person interested in understanding biology on a structural level and that would like to contribute to the field through lab work, methods d