the "production":
correct cloning, efficient transfection, titre of the virus, and play with
several parameters (temperature, cultivation of cells in suspension or
monolayer, day of harvesting of the cells, protein extraction procedure).
Good luck!
Alberto Manfrin
ESR Marie Curie
Hi Careina,
If your protein is expressed in E. coli, you can have a prediction of its
solubility on this website: http://biotech.ou.edu
Hope it helps!
Alberto Manfrin
From: Careina Edgooms
Reply-To: Careina Edgooms
Date: Tue, 22 Jan 2013 04:22:44 -0800
To:
Subject: [ccp4bb] protein