Re: [ccp4bb] Insect cell SF9 expression

2013-12-07 Thread Alberto Manfrin
the "production": correct cloning, efficient transfection, titre of the virus, and play with several parameters (temperature, cultivation of cells in suspension or monolayer, day of harvesting of the cells, protein extraction procedure). Good luck! Alberto Manfrin ESR Marie Curie

Re: [ccp4bb] protein solubility predictions

2013-01-23 Thread Alberto Manfrin
Hi Careina, If your protein is expressed in E. coli, you can have a prediction of its solubility on this website: http://biotech.ou.edu Hope it helps! Alberto Manfrin From: Careina Edgooms Reply-To: Careina Edgooms Date: Tue, 22 Jan 2013 04:22:44 -0800 To: Subject: [ccp4bb] protein