Hi Andre,
I recently prepared a Consurf-like figure using conservation scores pulled
from alignments in JalView. You would follow instructions for running
data2bfactor.py in PyMOL, feeding the conservation scores to replace your
model's B factor column. Then set up a color ramp like you would use
Along the lines of mass spec analysis, but probably more accessible to
many, is gel electrophoresis. Rinsing enough crystals and dissolving them
in your protein buffer should give you a sample suitable for standard
agarose and polyacrylamide gel electrophoresis protocols. I showed that
crystals I
Hi Careina,
One thing I'd mention in addition to the spot-on replies so far is that
cryoprotection strategies can influence diffraction. These look like pretty
spherulites though, so best of luck in optimization!
Andrew
On Wed, Nov 8, 2023, 10:03 AM careinaedgo...@yahoo.com <
