check this article its quite useful
http://www.ncbi.nlm.nih.gov/pubmed/12711344
On Wed, Jul 4, 2012 at 4:09 PM, amro selem wrote:
> hallo every body, i face problem with protein when i make gel filtration
> or concentrate my protein . i notice sever aggregation . i use only
> glycerol as additi
Crushing the crustal in mother liquor and serial dilution and seeding may
help in size improvement.
On Wed, Jul 4, 2012 at 3:03 PM, REX PALMER wrote:
> Does anyone have any new tips/methods for improving crystal size once the
> initial conditions have been established?
>
> Rex Palmer
> http://w
Dear all,
i am quite new to refinement and after refinement using refmac i have a
resonable R-factor and R-free (22 and 27%), but Molprobity analysis shows
c-beta devaition s as 23.
How can i fix this?
--
B. Anuradha
Research scholar,
CAS in crystallography and Biophysics,
University of Madras.
Dear all,
I have a problem . i crystallized same protein in two different pH and when
viewing the results a important beta hairpin seems extended in PyMOL .
(image link below).
https://picasaweb.google.com/anu4884/August222011?authuser=0&feat=directlink
But analysis of hairpin in promotif shows th
