Re: [ccp4bb] Crystal structures of phosphorylated proteins

2010-11-10 Thread Atlanta Cook
Perhaps you might pose a more directed question... Do you want to produce phosphorylated protein? Are you having trouble crystallising a protein that is phosphorylated? Atlanta On 10 Nov 2010, at 11:09, Sally Pham Thanh Van wrote: Dear all, Could you please tell me any information reg

Re: [ccp4bb] smeared bacteria pellets in protein expression

2010-05-12 Thread Atlanta Cook
We have had problems with phage contamination, either through contaminated plasmid stocks or more sporadic infections. Even if the cultures don't look clear, you can usually spot the contamination if the E. coli pellets are slimy and spread all the way up the centrifuge bottles. We have had

Re: [ccp4bb] problem with CCP4i - problem solved

2009-08-25 Thread Atlanta Cook
/share/ccp4i/etc/UNIX and edit my configure.def file. Almost at the end of the file, there is a line that says "USE_DBCCP4I_ON_STARTUP". Change the value from 1 to 0. This should "refresh" your project directories in the ccp4 GUI. HTH- Brad On Tue, Aug 25, 2009 at 9

[ccp4bb] problem with CCP4i

2009-08-25 Thread Atlanta Cook
Hi, I am running CCP4 version ccp4-6.1.1 on Mac OS X. I was working without any problems until the gui crashed and when I tried to start it up again it lost my PROJECT directory information. I restarted the computer and now I can't even get the gui up, if flashes up for a second and the