Dear all,
Our group (Institute of Molecular Medicine - Center for Translational Cancer
Research of UTHealth at Houston) is looking to hire a research
scientist/postdoc immediately with experience in protein chemistry, protein
engineering, and/or Bioconjugation.
Our group is working on
Hi all
Merry Christmas!!
Sorry for not ccp4 question.
I have a problem about multi-crystals averaging by using PHENIX 1.4-3
version.
I have two crystals. one is low resolution crystal form I(3.6A P6422;
monomer) another is crystal form II(3.1A P42212; dimer).
I did MAD phasing by using
Dear all,
I have a refinement problem.
I used a perfect twinning structure(data set processed with P21212, supected
pseudo-symmetry and expanded the data to P2. twnning parameter used the
refinement)
as a search model to do MR. After MR I can get a Rfac 0.52 structure. I am
refining the
HI all,
Thank you very much for your kindly answer.
I also tried to use different program like phaser, amore, and some MR servers.
actualy, I don`t know how many molecule in AU clearly. 2-6 molecules in 76%-
35% of solvent content. 4mer is 50% of solvent content. the homolgous
structure has
that it is
correct solution. I checked the solution and found some clashes in the
structure.I am not sure why the Rfactor too closed in next the molecule
search? I know this is unusual solution by molrep. Does it mean the
diffraction data has problem?
Any suggestions are welcome.Thank you in advance!
Carl
.
Make the third run inputting both of these peak values. Give NMON as 4.
You can also use the protein sequence hare.
Then refine it with Refmac, that again should decrease your R factor.
I will be highly benefitted if some one point out my mistake.
regards
Debajyoti
On Fri, 25 Jul 2008 Carl Soja
