Re: [ccp4bb] Patterson problem

Hi Roxanne, Sorry, I should have replied yesterday. CCT389545 is one example of S710. I was just looking at these series structures and decided to ask you if you had something for DHX15. Best regards Catarina Dr Catarina Rodrigues | Senior Scientific Officer Hit Discovery and Structural

Re: [ccp4bb] drawing topology digram

Hi Zhang, You can use TopdDraw from ccp4. HTH Catarina -- Catarina Rodrigues, PhD Student Molecular Transport Signalling AFMB UMR 7257 CNRS/UI/UII Case 932 163 Avenue de Luminy 13288 Marseille cedex 9 (France) Tel : +33 04 91 82 55 60 Fax : 04 91 26 67 20 e-mail:catarina.rodrig...@afmb.univ

Re: [ccp4bb] expression of membrane proteins as GST fusions

Hi, To purify your protein you can use GST-Trap HP column, it is quite easy, you have to elute in a buffer with 20mM reduced glutathione. HTH, Catarina -- Catarina Rodrigues, PhD Student Molecular Transport Signalling AFMB UMR 6098 CNRS/UI/UII Case 932 163 Avenue de Luminy 13288 Marseille

Re: [ccp4bb] Dehydration treatments

Dear Israel, This paper describes a procedure to crystal desiccation that allowed the improvement of diffraction: C. Abergel Acta Cryst. (2004). D60, 1413-1416 http://scripts.iucr.org/cgi-bin/paper?S0907444904013678 HTH Good luck, Catarina -- Catarina Rodrigues, PhD Student Molecular