Dear all,
I am refining the X-ray structure of a protein:
Data to ~2A were collected at a latest-generation synchrotron.
The 2fo-Fc maps are crisp, the model of the protein is complete and I am
reasonably happy with the stats (R below 20%, Rfree below 25% in Refmac 5.5).
However, I am seeing a
Dear all,
Thank you to all those who responded to my question about freezing crystals
grown in isopropanol.
To recap:
- my crystals grew in 25% isopropanol, plus some citrate buffer at pH4.5 (no
other ingredients).
- my crystals grew at room temperature, in 1micro-litre sitting drops, in
Dear all,
I have a protein which crystallizes in 25% isopropanol, at pH4.5.
Does anyone have experience freezing crystals grown in such a condition?
What cryoprotectants should I try?
Can isopropanol itself act as a cryoprotectant?
Any suggestions on how to deal with isopropanol evaporation
