Re: [ccp4bb] TEV vs HRV3C

2022-12-07 Thread Crissy L Tarver
We use the HRV3C protease site frequently in our lab. It provides better on-bead/column cleavage than TEV. We have a plasmid as we make 3C protease in our lab and keep stocks in our -80C. I can send you our protocol if you wish. Crissy Crissy L Tarver, PhD Department of Structural Biology

Re: [ccp4bb] OFF TOPIC question

2020-11-26 Thread Crissy L Tarver
BL21(DE3) pLysS using 5X KCM and heat shock transformation worked for me. Crissy L Tarver Postdoctoral Researcher Department of Structural Biology Stanford University School of Medicine From: CCP4 bulletin board on behalf of Hughes, Jonathan Sent: Thursday

Re: [ccp4bb] Macromolecular Crystallography workshop in South America 2020

2020-02-05 Thread Crissy L. Tarver
;> >> >>>> Applicants: >> >>>> 25 students will be selected, prioritizing advanced PhD, postdocs >> >>>> and young researchers. The Course will provide financial support >> >>>> covering registration fees, and for the case of those students >> >&g

Re: [ccp4bb] ORCID

2019-08-19 Thread Crissy L. Tarver
the CCP4BB list, click the following link: > https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1 > -- Crissy L. Tarver, Doctoral Candidate Biotechnology Science & Engineering Department of Biological Sciences University of Alabama Huntsville Success is not the key to happiness