We use the HRV3C protease site frequently in our lab. It provides better
on-bead/column cleavage than TEV. We have a plasmid as we make 3C protease in
our lab and keep stocks in our -80C. I can send you our protocol if you wish.
Crissy
Crissy L Tarver, PhD
Department of Structural Biology
BL21(DE3) pLysS using 5X KCM and heat shock transformation worked for me.
Crissy L Tarver
Postdoctoral Researcher
Department of Structural Biology
Stanford University School of Medicine
From: CCP4 bulletin board on behalf of Hughes, Jonathan
Sent: Thursday
;>
>> >>>> Applicants:
>> >>>> 25 students will be selected, prioritizing advanced PhD, postdocs
>> >>>> and young researchers. The Course will provide financial support
>> >>>> covering registration fees, and for the case of those students
>> >&g
the CCP4BB list, click the following link:
> https://www.jiscmail.ac.uk/cgi-bin/webadmin?SUBED1=CCP4BB&A=1
>
--
Crissy L. Tarver, Doctoral Candidate
Biotechnology Science & Engineering
Department of Biological Sciences
University of Alabama Huntsville
Success is not the key to happiness
