Re: [ccp4bb] how to optimize crystallization of a membrane proteinf

2010-08-31 Thread Damon Colbert
I have had a lot of problems of my own with poorly diffracting (or not at all) membrane protein crystals. After a previous discussion here, I summarised the suggestions I got in this ccp4 user wiki; http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Improving_crystal_quality There are

Re: [ccp4bb] linux question

2010-02-28 Thread Damon Colbert
I have a persistent Kubuntu 8.10 on a 16Gb flash drive, on which I have been running the likes of Coot, iMosflm and CCP4. I have used my setup to process two datasets via MolRep. There are plenty of resources on the internet telling you how to set it up. e.g http://www.pendrivelinux.com/ http

Re: [ccp4bb] SUMMARY: Poor diffraction of eukaryotic membrane protein crystals

2009-02-12 Thread Damon Colbert
On request, this summary (slightly amended) has been posted to the CPP4 wiki Crystal Growth page. You can find it here: http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/Improving_crystal_quality Regards, Damon. Damon Colbert schrieb: > > Thanks to everyone who responded wit

[ccp4bb] SUMMARY: Poor diffraction of eukaryotic membrane protein crystals

2009-02-11 Thread Damon Colbert
n in presence of ligand. * Attempt to collect a 10Ang dataset and try MR with a close homolog. Many thanks. Regards, Damon. ______ Damon Colbert School of Biological Sciences University of Auckland Email: d.colb...@auckland.ac.nz

[ccp4bb] Poor diffraction of eukaryotic membrane protein crystals

2009-02-04 Thread Damon Colbert
off-topic request for advice. Regards, Damon. __ Damon Colbert School of Biological Sciences University of Auckland Email: d.colb...@auckland.ac.nz3