Have you considered a Raspberry Pi with camera, or camera mount with
your existing lens:
https://www.raspberrypi.com/products/#cameras-and-displays
Photos and livestream will be easy to set up. You could try motioneye to
give you a livestream and take pictures on demand, or at defined time
The pACYC vectors (probably your p15ori plasmid) is said to have a copy
number of ~10 copies per cell while your other plasmid is likely in the
hundreds - see:
https://blog.addgene.org/plasmid-101-origin-of-replication
The protein expression level from the lower copy number plasmid is
Structure of 2019-nCov RNA polymerase:
https://www.biorxiv.org/content/10.1101/2020.03.16.993386v1.full.pdf+html
/Here we report the cryo-EM structure of 2019-nCoV full-length nsp12 in
complex with cofactors nsp7 and nsp8 at a resolution of 2.9-Å...A
comparative analysis to show how
Hello,
You could run Image Quant TL in a VM (parallels, vmware or virtual box).
https://bmi.cchmc.org/resources/software/imagequant-tl
For this appliation (arrays), we use an old program called VisualGrid
that is no longer available and run it an isolated XP VM via virtual box
(in linux).
Influenza polymerase heterotrimer expressed in insect cells (MultiBac
technology) as a fully synthetic polyprotein with subunits separated by
TEV sites
TevProtease-Tev-PA-Tev-PB1-Tev-PB2-Tev-CFP
Reich et al. Nature 2014; doi:10.1038/nature14009
/Extended Data Figure 1 | Production and
ntific Linux 7 (which means
it should also work on RHEL7 and Centos 7)
Thank you.
On 5/16/19 10:47 AM, Darren Hart wrote:
Some more info:
https://snapcraft.io/pymol-oss
It seems to work exactly as expected.
Darren
On 16/05/2019 16:05, Folmer Fredslund wrote:
Hi Darren,
That's brilliant!
Some more info:
https://snapcraft.io/pymol-oss
It seems to work exactly as expected.
Darren
On 16/05/2019 16:05, Folmer Fredslund wrote:
Hi Darren,
That's brilliant!
I'll give it a spin and see how it works.
Best regards
Folmer
tor. 16. maj 2019 13.02 skrev Darren Hart <mailto:darre
Since yesterday, PyMOL (open source version v2.3) has been packaged as a
distro-independent "snap" that can be installed easily on linux
platforms - no more cloning from gitlab and compiling after installing
the dependencies.
On Ubuntu, install from software centre or:
sudo snap install
*Announcement of the iNEXT Annual User Meeting in Grenoble*
The* 3**^rd **Annual Users Meeting of iNEXT* will take place from
*19*^*th * *to 21*^*st* * March 2018*at the European Photon and Neutron
(EPN) Science campus in *Grenoble (France)*. The EU-funded structural
biology project *iNEXT*
Set up a certificate via letsencrypt and move to https? It is quick,
easy and free.
https://letsencrypt.org/
Darren
On 11/10/17 18:34, Edward A. Berry wrote:
Is it because of this? Its been coming for a while now:
https://blog.mozilla.org/security/2015/04/30/deprecating-non-secure-http/
I
Adapted from periplasmic fractionation protocol, but for cytoplasmic
proteins:
1. Incubate resuspended cells with lysozyme in normal buffer but with
20% sucrose.
2. Spin down sphaeroplasts (unbroken cells with no cell wall - ultra
fragile). This also removes periplasmic proteases.
3.
Try
http://gentle.magnusmanske.de/
Resembles VectorNTI, is free, and works on Linux, Windows and Mac
(although I've only used it on XP).
Seems quite powerful, but not polished like the more commercial solutions.
Darren
On 08/02/13 05:16, Theresa Hsu wrote:
Dear all
Is there any good
), Hinxton (UK) and Monterotondo (Italy).
A technician position is available in the High Throughput Protein
Technologies laboratory of Darren Hart at EMBL Grenoble. It is funded by
P-CUBE and BioStruct-X, European “Research Infrastructures” projects
established to provide access to unique high-throughput
Workshop on Advanced Protein Engineering Techniques 3rd P-CUBE User
Meeting Heidelberg, 22 - 24 October 2012*
*
* Please apply here: www.p-cube.eu*
Registration deadline: 10 August 2012
*3rd P-CUBE User Meeting (22 Oct 2012)*
Get to know everything about the numerous platforms of P-CUBE and
, and will be sure to post a summary for future
reference once this issue is solved.
Sincerely,
--
J. Valencia S.
PhD student
CGR-NU
--
**
Dr. Darren Hart,
Team Leader
High Throughput Protein Lab
Grenoble Outstation
to prevent the insertion of mutations. So I have no idea
about what may be the problem.
I hope you could help me. Thank you.
Best regards,
--
---
Rubén Sánchez
--
**
Dr. Darren
. The organization
accepts no liability for any damage caused by any virus transmitted by this
email.
=
--
**
Dr. Darren Hart,
Team Leader
High Throughput Protein Lab
Grenoble Outstation
European
, but perhaps someone has
made one for private use.
Cheers,
Robbie Joosten
Biochemistry
Netherlands Cancer Institute
--
**
Dr. Darren Hart,
Team Leader
High Throughput Protein Lab
Grenoble Outstation
European Molecular
, allowing 3 universities to benefit from its use.
I’ve used schedule it software which is available as open source.
http://www.php.brickhost.com/
hope it’s of use,
best regards,
Paul.
--
**
Dr. Darren Hart,
Team Leader
unused time if the sample prep fails.
Any suggestions would be greatly appreciated. If governments can be toppled
through Facebook, I'm sure we can book our Aktas in a better way!
Darren
--
**
Dr. Darren Hart,
Team Leader
High
*
#
--
**
Dr. Darren Hart,
Team Leader
High Throughput Protein Lab
Grenoble Outstation
European Molecular Biology Laboratory (EMBL)
**
www.embl.fr/research/unit/hart/index.html
For funded access to ESPRIT construct
for your suggestions,
Darren
--
**
Dr. Darren Hart,
Team Leader
High Throughput Protein Lab
Grenoble Outstation
European Molecular Biology Laboratory (EMBL
. Darren Hart,
Team Leader
High Throughput Protein Lab
Grenoble Outstation
European Molecular Biology Laboratory (EMBL)
**
EMBL webpages:
http://tinyurl.com/6xdltl
http://tinyurl.com/667jrp
Email: h...@embl.fr
Tel: +33 4 76 20 77 68
object to the
strategy employed here on this occasion. Hence my original post to find out
what the alternatives are.
Darren
2009/1/30 Jeffrey Wilson wil...@ucmail.uc.edu
On Jan 28, 2009, at 3:47 AM, Darren Hart wrote:
ps anyone using VNTI might consider a backup of their work by exporting
files
Hello,
After several years of offering the molecular biology software VectorNTI
free to the academic community (their open access program) and building
up a huge user base, Invitrogen have suddenly announced that they will no
longer renew these free licences and the existing ones will be left to
that are difficult to use for sequence
manipulation. As a lab with hundreds of constructs and primers in our
database, we also appreciate the file arrangement/storage as well as the
sequence analysis function.
Thanks
Darren
2009/1/28 Darren Hart h...@embl.fr
Hello,
After several years of offering
Nordlund Lab, Karolinska Institute, Sweden
Renos Savva Domainex Ltd, UK
Darren Hart EMBL Grenoble, France
Martin WalshMRC France, Grenoble
Amir AharoniWeizmann Institute, Israel
Sabine Mazaleyrat AstraZeneca (ex), UK
Chris Meier UCB
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