Dear Mark, I have had quite a lot of success by adding small molecules during cell lysis to aid in solubility. From screening 100+ condition we found trehalose, betaine and mannitol to be the standouts with aiding in solubility.
Here is the relevant paper: http://www.ncbi.nlm.nih.gov/pubmed/23285060 David On Tue, Feb 26, 2013 at 2:08 AM, Mark J van Raaij <mjvanra...@cnb.csic.es>wrote: > Dear All, > > We are considering trying additives to bacterial cultures to see if they > improve solubility of some proteins we express: L-arginine, glycyl-glycine, > sorbitol and betaine. I would be glad to receive recommendations and > experiences, also if they haven't worked. > Side-question: should we get cell culture-certified compounds, or would > "normal", much more economical ones suffice? (i.e. are they likely to > contain side-products that would affect bacterial growth?). > > Greetings, > > Mark > > Mark J van Raaij > Lab 20B > Dpto de Estructura de Macromoleculas > Centro Nacional de Biotecnologia - CSIC > c/Darwin 3 > E-28049 Madrid, Spain > tel. (+34) 91 585 4616 > http://www.cnb.csic.es/~mjvanraaij > > -- David J. Leibly Graduate Student, Yeates Lab Dept. Chem & Biochem Univ. of California, Los Angeles Boyer Hall 269B (310) 825-8901