Re: [ccp4bb] TEV vs HRV3C

Hi All, I agree with everything said and I also find 3C, my first choice, to cleave more efficiently/rapidly. But I remember all the folks working with membrane proteins were using TEV, so I wonder whether TEV may be more resistent to detergent? (but I never read/found any info about this).

Re: [ccp4bb] off-topic: experience BioRad NGC vs Aekta Pure

Hi Oleksiy, If the Akta Start is just for loading affinity columns (without air sensor or software), why not to get a Cyvita peristaltic pump for £3k rather than £9k? They

Re: [ccp4bb] ligand binds to one molecule

I dont think it is necessary to prove what others have said so far, but if you would like some concrete evidence to support those statements: we cocrystallized a protein together with a small peptide, producing a crystal with for molecules in the AU but only one promoter showed clear density

Re: [ccp4bb] SHELXD for help

Hi Fu, "The SCALEn (n = 1, 2, or 3) records present the transformation from the orthogonal coordinates as contained in the entry to fractional crystallographic coordinates." (please see here https://www.wwpdb.org/documentation/file-format-content/format33/sect8.html) "The self-correlation

Re: [ccp4bb] freezing in ethane / propane - unipucks ?

Dear Guenter, Would the use of vials inside combi-pucks (https://www.mitegen.com/product/combipuck-system/) and some arrangements with your local contact at the other end, perhaps help with using propane remotely? BW, D On 26/01/2022 16:53, Guenter Fritz wrote: Dear all, we have some 

Re: [ccp4bb] Improved support for extended PDBx/mmCIF structure factor files

) - scaled unmerged data (MTZ or XDS_ASCII) to this converter: https://project-gemmi.github.io/wasm/mxdepo.html and then you can upload the resulting cif file to the wwPDB deposition system. Best wishes Marcin On Thu, 13 Jan 2022 at 20:34, Dom Bellini - MRC LMB wrote: Dear Jasmine, But can we now

Re: [ccp4bb] Improved support for extended PDBx/mmCIF structure factor files

Dear Jasmine, But can we now upload two mtz/mmcif files now for the structure factors? as in, the one from the processing software containing the raw data, as well as the one with the weighted amplitudes and the phases from Refinement software? Or do we still have to use CAD to create a

Re: [ccp4bb] [External] [ccp4bb] High-order oligomers vs robust crystals - references?

Hi Frank, Once we could not crystallize a monomer protein, but we knew the structure of a homolog dimer. We made some educated guessed mutations to dimerize our monomer target, which were based on the structure of the dimeric homolog. Perhaps evidence or perhaps just luck? :-)

Re: [ccp4bb] Problems with refining covalently linked heme cofactor

Dear Vera, In my experiences I had no problems to link protein atoms to a ligand in Coot. Did you check that you merged the cofactor to the protein molecule first? otherwise that could be one reason why Coot is not creating the link. BW, D On 13/07/2021 14:33, Vera Pfanzagl wrote: Hello,

Re: [ccp4bb] unknown density in 1.6 A structure

Hi Peer, If I understand you correctly you are saying that you do not know what substance may be in the density; then perhaps you only need to extend by an extra atom to create a new kind of symmetrical molecule that will fit the density? BW, D On 03/06/2021 11:55, SHEPARD William wrote: