*Position Description: * The Toth lab at the Institute for Bioscience and Biotechnology Research is seeking a highly motivated postdoctoral fellow for an NIH-funded research program aimed at developing designer proteases that selectively destroy active RAS (see Chen, Y. and Toth, E.A., et al., 2021, *Communications Biology*, *4*, 299). The overall objective of the research program is to develop general principles for engineering proteases that can control signaling in cells, with the RAS family of proteins as our current proof-of-principal target. The Postdoctoral Fellow will be involved in research projects involving engineering of candidate RAS-specific proteases, analysis of protease-substrate complexes, and analysis of the activity and effects of the proteases in cell models. The Postdoctoral Fellow will be expected to independently design and apply molecular biology, biochemical, and structural biology tools for the expression, characterization, and structure determination of novel RAS-specific proteases.
The successful candidate will be able to generate purified recombinant RAS-specific proteases and RAS proteins, characterize those proteins and their complexes, and determine the structures of protease and protease-substrate complexes. The individual will also analyze the effectiveness of the RAS-specific proteases in controlling RAS signaling and cell proliferation in cancer cell models harboring oncogenic RAS mutants using established cell-based assay techniques. The individual will also provide technical direction to laboratory staff, assist the PI in data management, preparation of manuscripts, grant proposals, and facilitate operations of the laboratory. This position is funded through March 2025. *Qualifications:* The position requires a Ph.D. in biochemistry, molecular biology, or a closely related discipline. Candidates who have practical experience in molecular biology, protein chemistry and/or structural biology will be considered with the highest priority. The successful candidate must be highly motivated with expertise in the fields of structural biology or biochemistry with a focus on structure-function relationships. A particular research focus and interest in in structure determination by X-ray crystallography or cryoelectron microscopy is preferred. In particular, the individual should have extensive experience in biochemistry as used in the cloning, expression, purification, and characterization of recombinant proteins, crystallization screening, protein structure determination, and refinement. Experience in mammalian cell culture and cell-based assay techniques is preferred. *Salary:* Commensurate with qualifications -- *Eric A. Toth, Ph.D. * Research Assistant Professor Institute for Bioscience and Biotechnology Research 9600 Gudelsky Drive Rockville, MD 20850 Email: eat...@umd.edu Phone: x-240-314-6516 ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
