Dear All,
A reminder that the earlybird deadline for the course on Protein engineering by
the Biochemical Society is coming up on the 15th May.
For more information the link is here:
https://www.biochemistry.org/Events/tabid/379/MeetingNo/SA219/view/Conference/Default.aspx
Protein
I did this course last year and I would highly recommend it! Also it is not
just for PhD students or early postdocs, there were a few of us last year with
more experience and we also got a lot out of it, especially in the practical
data collection and processing part (in the safe hands of
All great suggestions and a few new ones I haven't used before (thanks Emmanuel
and Frank).
I agree with Emmanuel, a fine screen should definitely be first port of call,
change the precipitant to go higher and lower than the initial hit (I normally
vary the PEG in 2% jumps, in a 24 or 48 well
Yes no images came through, however on looking at your crystallisation
conditions for clues, is there anything in the protein prep that could have
been carried through, even from the early stage buffers, or cell growth media?
We once had a detergent in the cell lysis stage that was removed from
I haven't used BSA, but I did recently use 50mM L-glutamic acid for this exact
reason (and 5% glycerol in all buffers except the last one for crystallography)
after reading this paper and it made a big difference to my last protein prep:
https://www.ncbi.nlm.nih.gov/pubmed/15264823
For my
