[ccp4bb] Senior Crystallography Position

Posted on behalf of Professor John Hunt and Professor Liang Tong, North Eastern Structural Genomics Consortium at Columbia University USA. An accomplished and dynamic senior scientist is sought to join the high-throughput protein biochemistry / crystallography group of the Northeast Structural G

Re: [ccp4bb] more on lattice translocation defects

There is also another paper on this that may be useful... Acta Cryst. (2005). D61, 67-74. Correction of X-ray intensities from single crystals containing lattice-translocation defects. J Wang et al. Regards -Original Message- From: CCP4 bulletin board [mailto:ccp...@jiscmail.ac.uk]

[ccp4bb] Off topic question

Hi CCP4ers Apologies for the off topic... I have been trying to get hold of L-ribulose-5-phosphate (D- is readily available). Does anyone know where I can get such a compound? Thanks in advance for any suggestions and Merry Xmas! Gina

Re: [ccp4bb] helix generator

I am not sure exactly if this is what you are asking but can't you just generate a alpha helix, in moleman, of the desired length, read that helix out and then change the ALA residues in that PDB to the ones you want? Gina On Jul 31, 2008, at 11:10 AM, Jacob Keller wrote: Sorry for bei

Re: [ccp4bb] Spooky, moving crystals

Hi Mark we also had a problem like this but in one case it was due to an instability in the goniometer head system so that the device still moved even after being "homed". Another few cases were due to an unstable magnetic base that was wobbling about due to the screw, on the gonimeter he

Re: [ccp4bb] Concentrating protein

r and Associate Consultant II Mayo Clinic Cancer Center Griffin Cancer Research Building, Rm 310 4500 San Pablo Road Jacksonville, FL 32224 (904) 953-6372 (office) (904) 953-0046 (lab) -Original Message- From: CCP4 bulletin board [mailto:[EMAIL PROTECTED] On Behalf Of Gina Clayton Sent: F

[ccp4bb] Disordered domains in crystal strutures

Dear CCP4ers can anyone recommend papers describing crystal structures of proteins with a large functionally important disordered domain or domains. Thanks in advance Gina

Re: [ccp4bb] Concentrating protein

Hi there I quite like the Amicon stirred ultra concentration cell systems. You can put large volumes in, maximum 1 litre size, I think. As well you can attach an inert gas such as Argon or Nitrogen, for the gaseous pressure, this reduces oxidation of your sample while it concentrates. M

Re: [ccp4bb] Bacterial induction at 18C

Raji aside from the possibilites of toxic protein as already mentioned.. we had great results with overnight induction at 20oC for a protein that was somewhat insoluble at 37oC. One thing that might work for you is to grow the cells to OD 0.5 then lower the temperature to say 18 or 20. After

Re: [ccp4bb] The importance of USING our validation tools

I thought that when a structure is deposited the databank does run its own refinement validation and geometry checks and gives you back what it finds i.e distance problems etc and rfactor? Quoting Eleanor Dodson <[EMAIL PROTECTED]>: The weighting in REFMAC is a function of SigmA ( plotted in l

[ccp4bb] density modification

Hi CCP4ers firstly apologies that this is slighty off ccp4 topic but does anyone know of a programme whereby I can use solvent flipping with multi domain masks in addition to a solvent mask for my phases i.e. not based on a model? I wanted to use CNS density_modify (my maps are currently from D