Please join us at our comprehensive single-particle cryo-EM workshop – Fundamentals of Single-Particle Cryo-EM – at the virtual American Crystallographic Association 71st annual meeting "Structural Science Awakens". This cryo-EM focused workshop will be held virtualy on 2 days: 8/17-18 from 12:15 to 5/6 pm ET and will focus on the fundamentals of cryo-EM, primarily benefiting those who are new to the field. In this workshop, we will highlight historical advances leading to the current state of the field, provide an overview of EM sample preparation and data collection, and detail cryo-EM data processing, interpretation and validation. We will monitor a live data collection and provide tutorials on “on-the-fly” cryo-EM data processing. This workshop is a companion workshop to “Managing and Using National cryo-EM Facilities”. Full workshop schedule is here: https://www.acameeting.com/workshops Elizabeth (Liz) Kellogg (Cornell), Ed Twomey (Johns Hopkins), and I, Mark Herzik (UC San Diego) have assembled a panel of leading cryo-EM experts to present practical, technical advice used in their own groups and to answer questions from the audience. Confirmed speakers include: Eva Nogales (UC Berkeley) Chris Russo (MRC) Mimi Ho (Columbia) Oliver Clarke (Columbia) Michael Cianfrocco (U. Michigan) Ed Eng (NCCAT) Craig Yoshioka (PNCC) Ali Punjani (Structura Biotechnology, CryoSPARC) As with past ACA workshops, this will be a highly interactive session with frequent interruptions from the audience and discussion concerning best practices among the panelists. A live demonstration of a data collection session at a national center will be included as well as “on-the-fly” live data processing using cryoSPARC Live. Fundamentals of single-particle cryo-EM Cryogenic-electron microscopy (cryo-EM) has emerged as an increasingly more powerful technique to visualize frozen biological macromolecules in near-native states. Recent advances have enabled cryo-EM to deliver high-resolution density maps approaching 1.5 Å or better with maps in the 2-4 Å range now routinely being attained, informing the construction of atomic models with high confidence. This new capacity has led to increasingly more interest in adopting cryo-EM and, consequently, there is a large need for training in cryo-EM principles and methods. Registration information for the ACA Meeting can be found here https://www.acameeting.com/registration-information. The ACA virtual meeting features the ACA M.J. Buerger Award winner, Wah Chiu, as well as sessions related to cryo-EM such as microED (1.1.3 Microcrystal electron diffraction), software development (2.1.3 Latest software developments in cryo-EM) and advances in detector technology (3.1.1) https://www.acameeting.com/schedule-sessions<https://urldefense.proofpoint.com/v2/url?u=https-3A__www.acameeting.com_schedule-2Dsessions&d=DwMFaQ&c=-35OiAkTchMrZOngvJPOeA&r=TFtVOfcgzix5ZmNyFhfxBw&m=4EyAODaGZPeoRBkk_L0kEUNQPFjV9Z3-dmsGveURlLM&s=_L1OgBN6EHrBey24Xgn31VP6bqF4JY11UAytEnY80tU&e=> Best wishes and we hope to see you there! Mark, Liz, and Ed
Thanks, -Mark Herzik, Ph.D. Assistant Professor Department of Chemistry and Biochemistry Natural Sciences Building 4105 University of California, San Diego 9500 Gilman Drive MC 0314 La Jolla, CA 92093 P: 858-246-5875 E: mherzik<at>ucsd.edu<http://ucsd.edu> herziklab.com<http://herziklab.com/> ######################################################################## To unsubscribe from the CCP4BB list, click the following link: https://www.jiscmail.ac.uk/cgi-bin/WA-JISC.exe?SUBED1=CCP4BB&A=1 This message was issued to members of www.jiscmail.ac.uk/CCP4BB, a mailing list hosted by www.jiscmail.ac.uk, terms & conditions are available at https://www.jiscmail.ac.uk/policyandsecurity/
