Hi,
In my hands, I have found the addition of TCEP (~0.5 mM) helps with TEV
cleavage efficiency, especially reactions at 4C where TEV is much less
efficient anyway. The caveat is that my targets may have been "happier" with
TCEP present, therefore encouraging more efficient cleavage. The
Hi,
Have you tried GESAMT? See
https://www.ccp4.ac.uk/html/gesamt.html#command-line-options
All the best,
Irwin
From: CCP4 bulletin board on behalf of Manoj N
Sent: Thursday, March 2, 2023 7:45 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Tools for
Hi all,
The SERp server appears to be down (https://services.mbi.ucla.edu/SER/). Does
anyone know of any alternatives or a perhaps a way to run something similar
locally?
Thanks,
Irwin
To unsubscribe from the CCP4BB
From: Marco Mazzorana
Sent: 22 December 2020 19:37
To: Irwin Selvam
Cc: CCP4BB@jiscmail.ac.uk
Subject: Re: [ccp4bb] Reprocessing images collected on an Eiger2 detector using
Xia2Dials on CCP4i2's GUI
Dear Irwin,
in addition to the very good suggestion by Dom, have you checked the Durin
plugin
Hi,
Unfortunately, the data were collected more than 30/40 days ago so I requested
them via TOPCAT - unless there is a way round this?
All the best,
Irwin
From: Dom Bellini
Sent: 22 December 2020 18:54
To: Irwin Selvam ;
CCP4BB@JISCMAIL.AC.UK
Subject: Re
Hi,
I'd like to reprocess some data that were collected on an Eiger2 detector at
Diamond with Xia2Dials using CCP4i2's GUI. I'm running CCP4i2 on a 64-bit
Windows 10 machine. The data were processed successfully at Diamond with no
obvious pathologies, the high resolution limit was just a
