er. Why not try a quantitative method (ITC, SPR)
> provided you have access of course.
>
> sincerely,
>
> Filip Van Petegem
>
>
>
> On Tue, Sep 21, 2010 at 12:01 PM, James Qiu wrote:
> Dear All,
>
> Firstly, sorry for the non-crystallography question but I
example
millimolar affinity, does that decrease the chances of co-crystallizing the two
interacting?
Thank you in advance,
Sincerely,
James Qiu
Hari,
I have expressed sulfite oxidase (with a N-terminal heme) in E. coli strain
TP1000 which resulted in red protein. I did not supplement the media with iron
or a porphyrin precursor.
Sincerely,
James
On Aug 26, 2010, at 12:56 PM, Hari Namboodiri wrote:
> Hi CCPers
>
> Can anyone provi
Dear Pranjal,
Some measure of a structure's "quality" compared to another is the number of
rotamer outliers, bad bond angles, ramachandran outliers and interpenetrating
atoms or "clashes" in a given structure. I would suggest using the Molprobity
website
http://molprobity.biochem.duke.edu/
r
