[ccp4bb] Job opening for a structural biologist

Job opportunity: Eternity Bioscience is hiring a structure biologist. Details please see ： http://www.eternitybioscience.com/contact-us -- Best regards, Joe

[ccp4bb] question about CCP4 scripts

Hi All, I am trying to write a shell script to streamline a few steps, one of which is Unique, see below. As you can see, this program requires symmetry and cell parameters. In CCP4 GUI Scalepack2mtz, these info are automatically extracted from .sca file (first two lines). But I don't know if

Re: [ccp4bb] advices

I guess you may have to decide whether you want to modify your current conditions or just start over to find new conditions, to get around this ultra-fast crystallization. I don't know if you have played around all parameters of current condition, such as lower temperature, protein concentration,

Re: [ccp4bb] protein degradation in crystal

Could you identify the cleavage sites by protein sequencing and design new constructs (truncated versions) accordingly? It might improve your crystal quality to get better resolution. Joe On Wed, Jan 16, 2013 at 9:22 AM, Tom Murray-Rust tom.murray.r...@gmail.comwrote: Just to add to

[ccp4bb] How to determine ligand binding from diffraction pattern?

Dear all, Is there a simple way to determine whether ligand is bound or not by comparing the diffraction patterns between ligand-free (structure known) and ligand-soaked protein crystals? I would like to solve the ligand bound protein structure, but before I do so, I have to find out if the