At 12:47 PM -0500 6/4/11, Jim Pflugrath wrote:
If any former participants in the course want to add to that description,
please feel free to respond to this thread.
...and, after a long day of learning and work, Girlie drinks for all!
- John
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At 9:47 AM -0700 4/9/11, Michael Thompson wrote:
Bradford dye binds to hydrophobic residues, mainly aromatics,
The statement above is not accurate.
Compton and Jones. Anal. Biochem. 151(2): 369-374, 1985
- John
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At 10:35 PM -0600 12/2/09, Dima Klenchin wrote:
2. Chances are good that you can use as high temperature as you need
to dissolve. I've come across protocols using =65C in the presence
of 100 mM bME to ensure complete unfolding of the polypeptide and
reduction of disulfides (seems essential
At 12:01 PM -0500 7/7/09, Jacob Keller wrote:
does anybody know of a simple, fast way to determine whether a
protein sample contains lipid, and roughly to determine the
quantity? As corollary, does anybody know of a quick way to
extract/remove the putative lipid? In my case, I am working with
