Hi Jerry,
Perhaps this Nature protocols paper from the Stroud Lab at UCSF might be useful.
Overexpressing human membrane proteins in stably transfected and clonal human
embryonic kidney 293S cells
http://www.nature.com/nprot/journal/v7/n3/full/nprot.2011.453.html
-john
On Aug 17, 2012, at
Hi Gloria,
Just to follow up Zhijie's email. We use E.coli expressed ULP1 protease
extensively in my lab, and it's very active and quite a bit cheaper than the
commercial version. My last prep from 2L produced greater than 50mg, and I've
more or less froze it directly off of the NiNTA elution.
Hi Anita,
One question that comes up immediately is why you are using DDM @ 5%
concentration. If it's a typo, understandable. I figure most of us use DDM at
0.05-0.1% concentration, and I've used as low as 0.015%.
Also, as others have pointed out, until you shoot it, you don't know. But if
i
A2220
Sigma
ANTI-FLAGĀ® M2 Affinity Gel
On Nov 2, 2010, at 11:01 AM, Oganesyan, Vaheh wrote:
> On the same note with Mirek: does anyone know of a source other than GE for
> resin for purification of FLAG-ed proteins?
>
> Thanks.
>
> Vaheh
>
> -Original Message-
> From: CCP4
The Stroud Lab at UCSF (http://www.msg.ucsf.edu/stroud/index.htm) is looking to
fill a post-doctoral position in membrane protein crystallography.
Applicants should have background in biochemistry, molecular biology, and/or
crystallography.
The position in membrane proteins will be in close a
letter and CV to:
beth...@msg.ucsf.edu
Please specify the position that you are applying to.
--
John K. Lee, Ph.D
University of California, San Francisco
Genentech Hall
600 16th. Street, S414
San Francisco, CA 94158-2517
USA