Re: [ccp4bb] best strategy to establish stable expression in mammalian cells

Hi Jerry, Perhaps this Nature protocols paper from the Stroud Lab at UCSF might be useful. Overexpressing human membrane proteins in stably transfected and clonal human embryonic kidney 293S cells http://www.nature.com/nprot/journal/v7/n3/full/nprot.2011.453.html -john On Aug 17, 2012, at

Re: [ccp4bb] SUMO(ULP-1) protease

Hi Gloria, Just to follow up Zhijie's email. We use E.coli expressed ULP1 protease extensively in my lab, and it's very active and quite a bit cheaper than the commercial version. My last prep from 2L produced greater than 50mg, and I've more or less froze it directly off of the NiNTA elution.

Re: [ccp4bb] detergent or protein

Hi Anita, One question that comes up immediately is why you are using DDM @ 5% concentration. If it's a typo, understandable. I figure most of us use DDM at 0.05-0.1% concentration, and I've used as low as 0.015%. Also, as others have pointed out, until you shoot it, you don't know. But if i

Re: [ccp4bb] on the same note

A2220 Sigma ANTI-FLAG® M2 Affinity Gel On Nov 2, 2010, at 11:01 AM, Oganesyan, Vaheh wrote: > On the same note with Mirek: does anyone know of a source other than GE for > resin for purification of FLAG-ed proteins? > > Thanks. > > Vaheh > > -Original Message- > From: CCP4

[ccp4bb] Post-doctoral Position in Membrane Protein Crystallography

The Stroud Lab at UCSF (http://www.msg.ucsf.edu/stroud/index.htm) is looking to fill a post-doctoral position in membrane protein crystallography. Applicants should have background in biochemistry, molecular biology, and/or crystallography. The position in membrane proteins will be in close a

[ccp4bb] Postdoctoral Positions UCSF/Stroud Lab

letter and CV to: beth...@msg.ucsf.edu Please specify the position that you are applying to. -- John K. Lee, Ph.D University of California, San Francisco Genentech Hall 600 16th. Street, S414 San Francisco, CA 94158-2517 USA