Re: [ccp4bb] 3D printing format

2015-05-14 Thread Kathleen Frey
Hi Joe, Here are some links that he might find helpful: https://www.thingiverse.com/thing:222918 https://www.thingiverse.com/thing:396459 >From the instructions on the second link: "The STL File of this protein was created using PyMOL, First, you download the PDB file from the protein databank

[ccp4bb] generating omit maps

2008-12-10 Thread Kathleen Frey
Hi Everyone, Can anyone tell me a relatively easy way to generate an omit density map for a ligand? I know that CNS can do this, but I was wondering if there's a CCP4 related program to generate omit maps. Thanks, Kathleen

[ccp4bb] Fwd: [ccp4bb] crystallization of proteins with His-tag and/or c-myc tags

2008-11-12 Thread Kathleen Frey
Returning to the previous discussion, we've had luck crystallizing several proteins with a His-tag. We've used both N-terminal and C-terminal his tags, and have had success with both of them. In one of our structures, the his tag actually formed an interface for crystal packing. We have also tried

[ccp4bb] ligand interaction viewers

2008-08-14 Thread Kathleen Frey
Hi Everyone, I've been using programs ligplot and ligand explorer to view protein-ligand and protein-cofactor interactions of a structure I've recently solved. Can anyone suggest a better program to view these interactions? Ligand explorer seems to only show distance (and not geometric) dependent

[ccp4bb] Alternate Ligand Conformations

2008-05-14 Thread Kathleen Frey
Hello. I am trying to refine a structure that has 2 ligand conformations as seen in the electron density. I tried to put both conformations in Coot and format the PDB similar to a residue alternate conformation and changing the occupancies to 0.50 for each conformation. This formatting did not wor