Keith's favourites over many years (shared with Gideon):
K2PtCl4
K2PtCN4
KAu(CN)2
Ethyl Mercury Thiosalicylate (gentle Hg reagent)
Try for about 2-12 hours at 1 mM initially. If crystals crack, reduce
concentration - at least they bind!
If these don't work, then finding a derivative is prob
Also, if you have a sample changer for easy screening, try 1 mM, 5 mM 20 mM of
each
for 1h, 5h, overnight, and screen all ~60 crystals for diffraction
overnight.Send those that survive
to the synchrotron.
K
On 15 Jan 2014, at 17:18, RHYS GRINTER wrote:
> Hello message board,
>
> My group ha
, followed
by protein crystallographic analysis.
The first is associated with the SPINE2COMPLEXES project on human
proteins.
The second is concerned with nucleotide metabolising proteins from
Campylobacter jejuni.
Please contact me for further details.
Keith Wilson
CTED])
if you have difficulty with the web site.
Keith Wilson
*
Prof. Keith S. Wilson,
Structural Biology Laboratory,
University of York,
YORK YO10 5YW
UK
tel 44-1904 328262
fax 44-1904 328266
1. SUBJECT: Postdoctoral Research Fellow in Structural Biology of Insulin
Postdoctoral Research Fellow in Structural Biology of Insulin, Insulin - like
Growth Factors. A postdoctoral position, funded by the Medical Research Council
within an international Programme Grant: A molecular dissection
Biological
Macromolecules
Società del Casino, Como, Italy
September 29th–October 3rd, 2008
ORGANISERS
Prof. Keith Wilson (York University, UK)
Dr. Marjolein Thunnissen (Lund University, Sweden)
Dr. Derek Logan (Lund University, Sweden)
AIMS
The school focuses on the application of X-ray
Biological
Macromolecules
Società del Casino, Como, Italy
September 29th–October 3rd, 2008
ORGANISERS
Prof. Keith Wilson (York University, UK)
Dr. Marjolein Thunnissen (Lund University, Sweden)
Dr. Derek Logan (Lund University, Sweden)
AIMS
The school focuses on the application of X-ray
