[ccp4bb] Emerald Plugmaker experiences?

2012-03-28 Thread Kothe, Michael GZ/US
Hi, I am looking for feedback from those who have used the Emerald Plugmaker instrument, especially if in your experience the translation of initial crystallization hits to diffracting crystals was as straightforward as claimed by Emerald. Any other thoughts and comments are also welcome. T

Re: [ccp4bb] protein stain, B-PER

2012-03-16 Thread Kothe, Michael GZ/US
Hi Artem, I have stayed away from these types of reagents in order to avoid detergents, and also because of anecdotal reports that these work less well for large-scale preps. Are you saying you use them routinely for preps of crystal-grade protein? Am I being too concerned about the presence of