s so much!
Ming
From: Jacob Keller [j-kell...@fsm.northwestern.edu]
Sent: Monday, August 27, 2012 9:31 AM
To: Lye, Ming
Cc: CCP4BB@jiscmail.ac.uk
Subject: Re: [ccp4bb] Off-topic: Duet vectors with C-terminal GST tag
How about just co-transfecting with two different plas
Dear CCP4bb,
We would like to co-express proteins under Se-Met conditions for de-novo
phasing of a complex. One of the proteins expresses much better with a GST-tag
compared with a his-tag.
As its binding site is at its N-terminus, we are hoping to co-express it as a
C-terminal GST tag recombi
Dear CCP4,
I am having problems running APBS in Pymol. Previous tries in which I took
the following steps worked, but for some reason, it's not running now even
though I'm following the exact same steps as previously and outlined
below:
Using the APBS plugin in Pymol:
1. Under 'Main', I select 'U
Dear CCP4 community,
Sorry for the off-topic subject, but I would really appreciate some
suggestions and/or protocols relating to native gel electrophoresis of
basic proteins. I have used a general acidic PAGE protocol for my protein,
which has a PI of 9.5. Briefly, the protein was loaded onto a n
